Manual therapy reduces pain behavior and oxidative stress in a murine model of complex regional pain syndrome type i

Afonso S.I. Salgado, Juliana Stramosk, Daniela D. Ludtke, Ana C.C. Kuci, Daiana C. Salm, Lisandro A. Ceci, Fabricia Petronilho, Drielly Florentino, Lucineia G. Danielski, Aline Gassenferth, Luana R. Souza, Gislaine T. Rezin, Adair R.S. Santos, Leidiane Mazzardo-Martins, William R. Reed, Daniel F. Martins

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Complex regional pain syndrome type I (CRPS-I) is a chronic painful condition. We investigated whether manual therapy (MT), in a chronic post-ischemia pain (CPIP) model, is capable of reducing pain behavior and oxidative stress. Male Swiss mice were subjected to ischemia-reperfusion (IR) to mimic CRPS-I. Animals received ankle joint mobilization 48h after the IR procedure, and response to mechanical stimuli was evaluated. For biochemical analyses, mitochondrial function as well as oxidative stress thiobarbituric acid reactive substances (TBARS), protein carbonyls, antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) levels were determined. IR induced mechanical hyperalgesia which was subsequently reduced by acute MT treatment. The concentrations of oxidative stress parameters were increased following IR with MT treatment preventing these increases in malondialdehyde (MDA) and carbonyls protein. IR diminished the levels of SOD and CAT activity and MT treatment prevented this decrease in CAT but not in SOD activity. IR also diminished mitochondrial complex activity, and MT treatment was ineffective in preventing this decrease. In conclusion, repeated sessions of MT resulted in antihyperalgesic effects mediated, at least partially, through the prevention of an increase of MDA and protein carbonyls levels and an improvement in the antioxidant defense system.

Original languageEnglish (US)
Article number197
JournalBrain Sciences
Volume9
Issue number8
DOIs
StatePublished - Aug 2019
Externally publishedYes

Keywords

  • Chronic pain
  • Complex Regional Pain Syndrome
  • Manual therapy
  • Osteopathy
  • Oxidative stress

ASJC Scopus subject areas

  • General Neuroscience

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