MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion

G. Martinez, C. Di Giacomo, M. L. Carnazza, V. Sorrenti, R. Castana, M. L. Barcellona, J. R. Perez-Polo, A. Vanella

Research output: Contribution to journalArticle

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Abstract

Plasticity in the central nervous system after cerebral ischemia is a controversial issue; focal cerebral ischemia produces an area of infarction that is surrounded by neurons that may respond to nearby damage by creating new synapses. In the present study the expression of the postsynaptic microtubule-associated protein 2 (MAP2) and the presynaptic marker protein, synaptophysin, was investigated by immunocytochemical techniques in the CA1 sector of hippocampus and in cerebellum of rats made ischemic by bilateral clamping of common carotid arteries and reperfused for 7 and 30 days. In addition, ischemia-induced behavioral alterations were also evaluated after 7 and 30 days of reperfusion. The present study demonstrates a decreased postsynaptic MAP2 immunoreactivity, representative of neuronal loss, particularly in CA1 sector of hippocampus and in cerebellum of ischemic rats reperfused for 7 days. After 30 days of reperfusion, MAP2 immunostaining was similar to control. In the same brain sections an increased presynaptic synaptophysin immunoreactivity has been observed only after 30 days of reperfusion. These data suggest compensatory regenerative changes associated with synaptic remodelling and are supported by behavioral recovery observed under the same experimental conditions.

Original languageEnglish (US)
Pages (from-to)457-464
Number of pages8
JournalDevelopmental Neuroscience
Volume19
Issue number6
StatePublished - Nov 1997
Externally publishedYes

Fingerprint

Synaptophysin
Microtubule-Associated Proteins
Reperfusion
Brain Ischemia
Cerebellum
Hippocampus
Brain
Common Carotid Artery
Constriction
Synapses
Infarction
Ischemia
Central Nervous System
Neurons
Proteins

Keywords

  • Behavior
  • Cerebral ischemia
  • MAP2
  • Synaptophysin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Martinez, G., Di Giacomo, C., Carnazza, M. L., Sorrenti, V., Castana, R., Barcellona, M. L., ... Vanella, A. (1997). MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion. Developmental Neuroscience, 19(6), 457-464.

MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion. / Martinez, G.; Di Giacomo, C.; Carnazza, M. L.; Sorrenti, V.; Castana, R.; Barcellona, M. L.; Perez-Polo, J. R.; Vanella, A.

In: Developmental Neuroscience, Vol. 19, No. 6, 11.1997, p. 457-464.

Research output: Contribution to journalArticle

Martinez, G, Di Giacomo, C, Carnazza, ML, Sorrenti, V, Castana, R, Barcellona, ML, Perez-Polo, JR & Vanella, A 1997, 'MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion', Developmental Neuroscience, vol. 19, no. 6, pp. 457-464.
Martinez G, Di Giacomo C, Carnazza ML, Sorrenti V, Castana R, Barcellona ML et al. MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion. Developmental Neuroscience. 1997 Nov;19(6):457-464.
Martinez, G. ; Di Giacomo, C. ; Carnazza, M. L. ; Sorrenti, V. ; Castana, R. ; Barcellona, M. L. ; Perez-Polo, J. R. ; Vanella, A. / MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion. In: Developmental Neuroscience. 1997 ; Vol. 19, No. 6. pp. 457-464.
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