Mapping a major gene for resistance to rift valley fever virus in laboratory rats

Catherine M. Busch, Ralph J. Callicott, Clarence J. Peters, John C. Morrill, James E. Womack

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The Rift Valley Fever virus (RVFV) presents an epidemic and epizootic threat in sub-Saharan Africa, Egypt, and the Arabian Peninsula, and has furthermore recently gained attention as a potential weapon of bioterrorism due to its ability to infect both livestock and humans. Inbred rat strains show similar characteristic responses to the disease as humans and livestock, making them a suitable model species. Previous studies had indicated differences in susceptibility to RVFV hepatic disease among various rat strains, including a higher susceptibility of Wistar-Furth (WF) compared to a more resistant Lewis (LEW) strain. Further study revealed that this resistance trait exhibits the pattern of a major dominant gene inherited in Mendelian fashion. A genome scan of a congenic WF.LEW strain, created from the susceptible WF and resistant LEW strains and itself resistant to infection with RVFV, revealed 2 potential regions for the location of the gene, 1 on chromosome 3 and the other on chromosome 9. Through backcrossing of WF.LEW rats to WF rats, genotyping offspring using SNPs and microsatellites, and viral challenges of 3 N1 litters, we have mapped the gene to the distal end of chromosome 3.

Original languageEnglish (US)
Pages (from-to)728-733
Number of pages6
JournalJournal of Heredity
Volume106
Issue number6
DOIs
StatePublished - 2015

Keywords

  • Gene mapping
  • Rats
  • Rift Valley Fever Virus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biotechnology
  • Genetics(clinical)

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  • Cite this

    Busch, C. M., Callicott, R. J., Peters, C. J., Morrill, J. C., & Womack, J. E. (2015). Mapping a major gene for resistance to rift valley fever virus in laboratory rats. Journal of Heredity, 106(6), 728-733. https://doi.org/10.1093/jhered/esv087