The mapping of contact surfaces in the monomer subunits by hydrogen/deuterium exchange and on-line HPLC electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was discussed. The CA protein existed in monomer-dimer equilibrium at low salt concentration and polymerized into long tube-like structures at high ionic strength. Recombinant CA protein was expressed in E. coli, the cells were lysed and capsid proteins were precipitated with ammonium sulfate. The results show that FT-ICR MS produces high primary protein sequence coverage (∼90%).
|Number of pages
|Published - Dec 1 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002 → Jun 6 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
|6/2/02 → 6/6/02
ASJC Scopus subject areas