Mapping of two dominant sites of VP35 of Marburg virus

A. V. Sorokin, E. I. Kazachinskaia, A. V. Ivanova, A. V. Kachko, S. V. Netesov, A. A. Bukreyev, V. B. Loktev, I. A. Razumov

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Five types of anti-VP35 monoclonal antibodies (MAbs), four immune sera against Marburg virus (MBGV), and 11 overlapping recombinant VP35 fragments were used to map the epitopes for VP35 of MBGV. The purified full-size recombinant VP35 was highly immunogenic and retained the B-cell epitopes that were identical to those of the viral VP35. Two major sites on VP35 and a set of truncated VP35 fragments were found by use of an enzyme immunoassay and immunoblot. Site I was located in a region between amino acids 1 and 174 of the VP35 sequence, and only polyclonal antibodies (PAbs) against MBGV recognized epitopes at this site. Site II was mapped by use of anti-VP35 MAbs to the region between amino acid residues 167 and 278 of VP35. Amino acids 252-278 of VP35 might be involved in the formation of the epitopes for MAbs. B-cell epitopes were not found on the C-terminus of VP35 by use of PAbs or MAbs.

Original languageEnglish (US)
Pages (from-to)481-492
Number of pages12
JournalViral Immunology
Volume15
Issue number3
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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    Sorokin, A. V., Kazachinskaia, E. I., Ivanova, A. V., Kachko, A. V., Netesov, S. V., Bukreyev, A. A., Loktev, V. B., & Razumov, I. A. (2002). Mapping of two dominant sites of VP35 of Marburg virus. Viral Immunology, 15(3), 481-492. https://doi.org/10.1089/088282402760312359