Mapping of two dominant sites of VP35 of Marburg virus

A. V. Sorokin; E. I. Kazachinskaia; A. V. Ivanova; A. V. Kachko; S. V. Netesov; A. A. Bukreyev; V. B. Loktev; I. A. Razumov

doi:10.1089/088282402760312359

Mapping of two dominant sites of VP35 of Marburg virus

A. V. Sorokin, E. I. Kazachinskaia, A. V. Ivanova, A. V. Kachko, S. V. Netesov, A. A. Bukreyev, V. B. Loktev, I. A. Razumov

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Five types of anti-VP35 monoclonal antibodies (MAbs), four immune sera against Marburg virus (MBGV), and 11 overlapping recombinant VP35 fragments were used to map the epitopes for VP35 of MBGV. The purified full-size recombinant VP35 was highly immunogenic and retained the B-cell epitopes that were identical to those of the viral VP35. Two major sites on VP35 and a set of truncated VP35 fragments were found by use of an enzyme immunoassay and immunoblot. Site I was located in a region between amino acids 1 and 174 of the VP35 sequence, and only polyclonal antibodies (PAbs) against MBGV recognized epitopes at this site. Site II was mapped by use of anti-VP35 MAbs to the region between amino acid residues 167 and 278 of VP35. Amino acids 252-278 of VP35 might be involved in the formation of the epitopes for MAbs. B-cell epitopes were not found on the C-terminus of VP35 by use of PAbs or MAbs.

Original language	English (US)
Pages (from-to)	481-492
Number of pages	12
Journal	Viral Immunology
Volume	15
Issue number	3
DOIs	https://doi.org/10.1089/088282402760312359
State	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Immunology
Molecular Medicine
Virology

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title = "Mapping of two dominant sites of VP35 of Marburg virus",

abstract = "Five types of anti-VP35 monoclonal antibodies (MAbs), four immune sera against Marburg virus (MBGV), and 11 overlapping recombinant VP35 fragments were used to map the epitopes for VP35 of MBGV. The purified full-size recombinant VP35 was highly immunogenic and retained the B-cell epitopes that were identical to those of the viral VP35. Two major sites on VP35 and a set of truncated VP35 fragments were found by use of an enzyme immunoassay and immunoblot. Site I was located in a region between amino acids 1 and 174 of the VP35 sequence, and only polyclonal antibodies (PAbs) against MBGV recognized epitopes at this site. Site II was mapped by use of anti-VP35 MAbs to the region between amino acid residues 167 and 278 of VP35. Amino acids 252-278 of VP35 might be involved in the formation of the epitopes for MAbs. B-cell epitopes were not found on the C-terminus of VP35 by use of PAbs or MAbs.",

author = "Sorokin, {A. V.} and Kazachinskaia, {E. I.} and Ivanova, {A. V.} and Kachko, {A. V.} and Netesov, {S. V.} and Bukreyev, {A. A.} and Loktev, {V. B.} and Razumov, {I. A.}",

year = "2002",

doi = "10.1089/088282402760312359",

language = "English (US)",

volume = "15",

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journal = "Viral Immunology",

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T1 - Mapping of two dominant sites of VP35 of Marburg virus

AU - Sorokin, A. V.

AU - Kazachinskaia, E. I.

AU - Ivanova, A. V.

AU - Kachko, A. V.

AU - Netesov, S. V.

AU - Bukreyev, A. A.

AU - Loktev, V. B.

AU - Razumov, I. A.

PY - 2002

Y1 - 2002

N2 - Five types of anti-VP35 monoclonal antibodies (MAbs), four immune sera against Marburg virus (MBGV), and 11 overlapping recombinant VP35 fragments were used to map the epitopes for VP35 of MBGV. The purified full-size recombinant VP35 was highly immunogenic and retained the B-cell epitopes that were identical to those of the viral VP35. Two major sites on VP35 and a set of truncated VP35 fragments were found by use of an enzyme immunoassay and immunoblot. Site I was located in a region between amino acids 1 and 174 of the VP35 sequence, and only polyclonal antibodies (PAbs) against MBGV recognized epitopes at this site. Site II was mapped by use of anti-VP35 MAbs to the region between amino acid residues 167 and 278 of VP35. Amino acids 252-278 of VP35 might be involved in the formation of the epitopes for MAbs. B-cell epitopes were not found on the C-terminus of VP35 by use of PAbs or MAbs.

AB - Five types of anti-VP35 monoclonal antibodies (MAbs), four immune sera against Marburg virus (MBGV), and 11 overlapping recombinant VP35 fragments were used to map the epitopes for VP35 of MBGV. The purified full-size recombinant VP35 was highly immunogenic and retained the B-cell epitopes that were identical to those of the viral VP35. Two major sites on VP35 and a set of truncated VP35 fragments were found by use of an enzyme immunoassay and immunoblot. Site I was located in a region between amino acids 1 and 174 of the VP35 sequence, and only polyclonal antibodies (PAbs) against MBGV recognized epitopes at this site. Site II was mapped by use of anti-VP35 MAbs to the region between amino acid residues 167 and 278 of VP35. Amino acids 252-278 of VP35 might be involved in the formation of the epitopes for MAbs. B-cell epitopes were not found on the C-terminus of VP35 by use of PAbs or MAbs.

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SP - 481

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JO - Viral Immunology

JF - Viral Immunology

IS - 3

ER -

Mapping of two dominant sites of VP35 of Marburg virus

Abstract

ASJC Scopus subject areas

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