Abstract
Sendai virus V protein is not essential for virus replication in cultured cells but is essential for efficient virus replication and pathogenesis in mice, indicating that the V protein has a luxury function to facilitate virus propagation in mice. This was discovered in the Z strain, an egg-adapted avirulent laboratory strain. In the present study, we reexamined the function of Sendai virus V protein by generating a V-knockout Sendai virus derived from the Hamamatsu strain, a virulent field isolate, which is an appropriate model for studying the natural course of Sendai virus infection in mice. We unexpectedly found that the V-knockout virus propagated efficiently in mice and was as virulent as the wild-type virus. Switching of the functionally important V unique region demonstrated that this region of the Hamamatsu strain was also functional in a Z strain background. It thus appears that the V protein is nonsense in a field isolate of Sendai virus. However, the V protein was required for virus growth and pathogenesis of the Hamamatsu strain in mice when the virulence of the virus was attenuated by introducing mutations that had been found in an egg-adapted, avirulent virus. The V protein therefore seems to be potentially functional in the highly virulent Hamamatsu strain and to be prominent if virus replication is restricted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 581-587 |
| Number of pages | 7 |
| Journal | Virology |
| Volume | 313 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1 2003 |
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Keywords
- Field isolate
- Pathogenesis
- Reverse genetics
- Sendai virus
- V protein
ASJC Scopus subject areas
- Virology
Cite this
Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate. / Sakaguchi, Takemasa; Kiyotani, Katsuhiro; Watanabe, Hitoshi; Huang, Cheng; Fukuhara, Noriko; Fujii, Yutaka; Shimazu, Yukie; Sugahara, Fumihiro; Nagai, Yoshiyuki; Yoshida, Tetsuya.
In: Virology, Vol. 313, No. 2, 01.09.2003, p. 581-587.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate
AU - Sakaguchi, Takemasa
AU - Kiyotani, Katsuhiro
AU - Watanabe, Hitoshi
AU - Huang, Cheng
AU - Fukuhara, Noriko
AU - Fujii, Yutaka
AU - Shimazu, Yukie
AU - Sugahara, Fumihiro
AU - Nagai, Yoshiyuki
AU - Yoshida, Tetsuya
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Sendai virus V protein is not essential for virus replication in cultured cells but is essential for efficient virus replication and pathogenesis in mice, indicating that the V protein has a luxury function to facilitate virus propagation in mice. This was discovered in the Z strain, an egg-adapted avirulent laboratory strain. In the present study, we reexamined the function of Sendai virus V protein by generating a V-knockout Sendai virus derived from the Hamamatsu strain, a virulent field isolate, which is an appropriate model for studying the natural course of Sendai virus infection in mice. We unexpectedly found that the V-knockout virus propagated efficiently in mice and was as virulent as the wild-type virus. Switching of the functionally important V unique region demonstrated that this region of the Hamamatsu strain was also functional in a Z strain background. It thus appears that the V protein is nonsense in a field isolate of Sendai virus. However, the V protein was required for virus growth and pathogenesis of the Hamamatsu strain in mice when the virulence of the virus was attenuated by introducing mutations that had been found in an egg-adapted, avirulent virus. The V protein therefore seems to be potentially functional in the highly virulent Hamamatsu strain and to be prominent if virus replication is restricted.
AB - Sendai virus V protein is not essential for virus replication in cultured cells but is essential for efficient virus replication and pathogenesis in mice, indicating that the V protein has a luxury function to facilitate virus propagation in mice. This was discovered in the Z strain, an egg-adapted avirulent laboratory strain. In the present study, we reexamined the function of Sendai virus V protein by generating a V-knockout Sendai virus derived from the Hamamatsu strain, a virulent field isolate, which is an appropriate model for studying the natural course of Sendai virus infection in mice. We unexpectedly found that the V-knockout virus propagated efficiently in mice and was as virulent as the wild-type virus. Switching of the functionally important V unique region demonstrated that this region of the Hamamatsu strain was also functional in a Z strain background. It thus appears that the V protein is nonsense in a field isolate of Sendai virus. However, the V protein was required for virus growth and pathogenesis of the Hamamatsu strain in mice when the virulence of the virus was attenuated by introducing mutations that had been found in an egg-adapted, avirulent virus. The V protein therefore seems to be potentially functional in the highly virulent Hamamatsu strain and to be prominent if virus replication is restricted.
KW - Field isolate
KW - Pathogenesis
KW - Reverse genetics
KW - Sendai virus
KW - V protein
UR - http://www.scopus.com/inward/record.url?scp=0041885418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041885418&partnerID=8YFLogxK
U2 - 10.1016/S0042-6822(03)00350-7
DO - 10.1016/S0042-6822(03)00350-7
M3 - Article
C2 - 12954223
AN - SCOPUS:0041885418
VL - 313
SP - 581
EP - 587
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -