Abstract
MS/MS of electrosprayed ions is shown to have the capacity to discriminate between peptides that differ by configuration about their α-carbons. It is not necessary for the peptides to possess tertiary structures that are affected by stereochemistry, since five epimers of the pentapeptide, H 2N-Gly-Leu-Ser-Phe-Ala-OH (GLSFA) all display different collisionally activated dissociation (CAD) patterns of their protonated parent ions. The figure of merit, r, is a ratio of ratios of fragment ion abundances between stereoisomers, where r = 1 corresponds to no stereochemical effect. Values of r as high as 3.8 are seen for diastereomer pairs. Stereochemical effects are also seen for the diprotonated dodecapeptide H2N-Leu-Val-Phe-Phe-Ala-Glu- Asp-Val-Gly-Ser-Asn-Lys-OH (LVFFAEDVGSNK), a tryptic fragment from the amyloid β-protein. Triply charged complexes of the protonated dodecapeptide with cobalt(II) ions undergo CAD at lower collision energies than do doubly protonated LWFAEDVGSNK ions. Statistically significant (p < 0.01) differences between the all-L-dodecapeptide and the ones containing a D-serine or a D-aspartic acid are observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5480-5487 |
| Number of pages | 8 |
| Journal | Analytical Chemistry |
| Volume | 77 |
| Issue number | 17 |
| DOIs | |
| State | Published - Sep 1 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Mass spectrometric differentiation of linear peptides composed of L-amino acids from isomers containing one D-amino acid residue. / Serafin, Scott V.; Maranan, Rhonda; Zhang, Kangling; Morton, Thomas Hellman.
In: Analytical Chemistry, Vol. 77, No. 17, 01.09.2005, p. 5480-5487.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mass spectrometric differentiation of linear peptides composed of L-amino acids from isomers containing one D-amino acid residue
AU - Serafin, Scott V.
AU - Maranan, Rhonda
AU - Zhang, Kangling
AU - Morton, Thomas Hellman
PY - 2005/9/1
Y1 - 2005/9/1
N2 - MS/MS of electrosprayed ions is shown to have the capacity to discriminate between peptides that differ by configuration about their α-carbons. It is not necessary for the peptides to possess tertiary structures that are affected by stereochemistry, since five epimers of the pentapeptide, H 2N-Gly-Leu-Ser-Phe-Ala-OH (GLSFA) all display different collisionally activated dissociation (CAD) patterns of their protonated parent ions. The figure of merit, r, is a ratio of ratios of fragment ion abundances between stereoisomers, where r = 1 corresponds to no stereochemical effect. Values of r as high as 3.8 are seen for diastereomer pairs. Stereochemical effects are also seen for the diprotonated dodecapeptide H2N-Leu-Val-Phe-Phe-Ala-Glu- Asp-Val-Gly-Ser-Asn-Lys-OH (LVFFAEDVGSNK), a tryptic fragment from the amyloid β-protein. Triply charged complexes of the protonated dodecapeptide with cobalt(II) ions undergo CAD at lower collision energies than do doubly protonated LWFAEDVGSNK ions. Statistically significant (p < 0.01) differences between the all-L-dodecapeptide and the ones containing a D-serine or a D-aspartic acid are observed.
AB - MS/MS of electrosprayed ions is shown to have the capacity to discriminate between peptides that differ by configuration about their α-carbons. It is not necessary for the peptides to possess tertiary structures that are affected by stereochemistry, since five epimers of the pentapeptide, H 2N-Gly-Leu-Ser-Phe-Ala-OH (GLSFA) all display different collisionally activated dissociation (CAD) patterns of their protonated parent ions. The figure of merit, r, is a ratio of ratios of fragment ion abundances between stereoisomers, where r = 1 corresponds to no stereochemical effect. Values of r as high as 3.8 are seen for diastereomer pairs. Stereochemical effects are also seen for the diprotonated dodecapeptide H2N-Leu-Val-Phe-Phe-Ala-Glu- Asp-Val-Gly-Ser-Asn-Lys-OH (LVFFAEDVGSNK), a tryptic fragment from the amyloid β-protein. Triply charged complexes of the protonated dodecapeptide with cobalt(II) ions undergo CAD at lower collision energies than do doubly protonated LWFAEDVGSNK ions. Statistically significant (p < 0.01) differences between the all-L-dodecapeptide and the ones containing a D-serine or a D-aspartic acid are observed.
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U2 - 10.1021/ac050490j
DO - 10.1021/ac050490j
M3 - Article
VL - 77
SP - 5480
EP - 5487
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 17
ER -