Mass spectrometry imaging of biological tissue

an approach for multicenter studies

Andreas Römpp, Jean Pierre Both, Alain Brunelle, Ron M.A. Heeren, Olivier Laprévote, Brendan Prideaux, Alexandre Seyer, Bernhard Spengler, Markus Stoeckli, Donald F. Smith

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Abstract Mass spectrometry imaging has become a popular tool for probing the chemical complexity of biological surfaces. This led to the development of a wide range of instrumentation and preparation protocols. It is thus desirable to evaluate and compare the data output from different methodologies and mass spectrometers. Here, we present an approach for the comparison of mass spectrometry imaging data from different laboratories (often referred to as multicenter studies). This is exemplified by the analysis of mouse brain sections in five laboratories in Europe and the USA. The instrumentation includes matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF), MALDI-QTOF, MALDI-Fourier transform ion cyclotron resonance (FTICR), atmospheric-pressure (AP)-MALDI-Orbitrap, and cluster TOF-secondary ion mass spectrometry (SIMS). Experimental parameters such as measurement speed, imaging bin width, and mass spectrometric parameters are discussed. All datasets were converted to the standard data format imzML and displayed in a common open-source software with identical parameters for visualization, which facilitates direct comparison of MS images. The imzML conversion also allowed exchange of fully functional MS imaging datasets between the different laboratories. The experiments ranged from overview measurements of the full mouse brain to detailed analysis of smaller features (depending on spatial resolution settings), but common histological features such as the corpus callosum were visible in all measurements. High spatial resolution measurements of AP-MALDI-Orbitrap and TOF-SIMS showed comparable structures in the low-micrometer range. We discuss general considerations for planning and performing multicenter studies in mass spectrometry imaging. This includes details on the selection, distribution, and preparation of tissue samples as well as on data handling. Such multicenter studies in combination with ongoing activities for reporting guidelines, a common data format (imzML) and a public data repository can contribute to more reliability and transparency of MS imaging studies.

Original languageEnglish (US)
Article number8410
Pages (from-to)2329-2335
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number8
DOIs
StatePublished - Mar 28 2015
Externally publishedYes

Fingerprint

Multicenter Studies
Mass spectrometry
Mass Spectrometry
Lasers
Ionization
Desorption
Tissue
Imaging techniques
Secondary Ion Mass Spectrometry
Atmospheric Pressure
Secondary ion mass spectrometry
Atmospheric pressure
Brain
Cyclotrons
Corpus Callosum
Fourier Analysis
Cyclotron resonance
Tissue Distribution
Data handling
Bins

Keywords

  • Data format imzML
  • Data handling and processing
  • Mass spectrometry imaging
  • Multicenter studies
  • Multimodal imaging

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Römpp, A., Both, J. P., Brunelle, A., Heeren, R. M. A., Laprévote, O., Prideaux, B., ... Smith, D. F. (2015). Mass spectrometry imaging of biological tissue: an approach for multicenter studies. Analytical and Bioanalytical Chemistry, 407(8), 2329-2335. [8410]. https://doi.org/10.1007/s00216-014-8410-7

Mass spectrometry imaging of biological tissue : an approach for multicenter studies. / Römpp, Andreas; Both, Jean Pierre; Brunelle, Alain; Heeren, Ron M.A.; Laprévote, Olivier; Prideaux, Brendan; Seyer, Alexandre; Spengler, Bernhard; Stoeckli, Markus; Smith, Donald F.

In: Analytical and Bioanalytical Chemistry, Vol. 407, No. 8, 8410, 28.03.2015, p. 2329-2335.

Research output: Contribution to journalArticle

Römpp, A, Both, JP, Brunelle, A, Heeren, RMA, Laprévote, O, Prideaux, B, Seyer, A, Spengler, B, Stoeckli, M & Smith, DF 2015, 'Mass spectrometry imaging of biological tissue: an approach for multicenter studies', Analytical and Bioanalytical Chemistry, vol. 407, no. 8, 8410, pp. 2329-2335. https://doi.org/10.1007/s00216-014-8410-7
Römpp, Andreas ; Both, Jean Pierre ; Brunelle, Alain ; Heeren, Ron M.A. ; Laprévote, Olivier ; Prideaux, Brendan ; Seyer, Alexandre ; Spengler, Bernhard ; Stoeckli, Markus ; Smith, Donald F. / Mass spectrometry imaging of biological tissue : an approach for multicenter studies. In: Analytical and Bioanalytical Chemistry. 2015 ; Vol. 407, No. 8. pp. 2329-2335.
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