TY - JOUR
T1 - Maternal protein restriction regulates IGF2 system in placental labyrinth
AU - Gao, Haijun
AU - Sathishkumar, Kunju Reddiar
AU - Yallampalli, Uma
AU - Balakrishnan, Meena
AU - Li, Xilong
AU - Wu, Guoyao
AU - Yallampalli, Chandra
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - This study was to test the hypothesis that altered IGF2 system in the placental labyrinth zone (LZ) impairs feto-placental growth in response to maternal protein restriction. Rats were fed a 20% protein diet and an isocaloric 6% protein diet (LP) from day 1 to days 14, 18, or 21 of pregnancy. The effects of diet, gender of placenta and fetus, and day of pregnancy on placental weight, fetal weight, and expression of the IGF2 axis in the placental LZ and amino acids in maternal plasma were analyzed. Growth restriction occurred in both female and male fetuses by LP, coincident with impaired LZ growth and efficiency. The expression of Igf2, Igf2P0, Igf1r, Igf2r, Insr, Igfbp1, and Igfbp2 in placental LZ were affected by diet, gender and/or day of pregnancy. Concentrations of total essential amino acids and total nonessential amino acids were reduced and increased, respectively, in maternal plasma of LP-fed rats. These results indicate that adaptation of the IGF2 system in rat LZ occurs in a sex- and time-dependent manner in response to maternal protein restriction; however, these adaptations cannot prevent the growth restriction of both male and female fetuses during late pregnancy.
AB - This study was to test the hypothesis that altered IGF2 system in the placental labyrinth zone (LZ) impairs feto-placental growth in response to maternal protein restriction. Rats were fed a 20% protein diet and an isocaloric 6% protein diet (LP) from day 1 to days 14, 18, or 21 of pregnancy. The effects of diet, gender of placenta and fetus, and day of pregnancy on placental weight, fetal weight, and expression of the IGF2 axis in the placental LZ and amino acids in maternal plasma were analyzed. Growth restriction occurred in both female and male fetuses by LP, coincident with impaired LZ growth and efficiency. The expression of Igf2, Igf2P0, Igf1r, Igf2r, Insr, Igfbp1, and Igfbp2 in placental LZ were affected by diet, gender and/or day of pregnancy. Concentrations of total essential amino acids and total nonessential amino acids were reduced and increased, respectively, in maternal plasma of LP-fed rats. These results indicate that adaptation of the IGF2 system in rat LZ occurs in a sex- and time-dependent manner in response to maternal protein restriction; however, these adaptations cannot prevent the growth restriction of both male and female fetuses during late pregnancy.
KW - Fetal Growth
KW - Gene Expression
KW - IGF2
KW - IGF2P0
KW - IGFBP
KW - Labyrinth Zone
KW - Placenta
KW - Pregnancy
KW - Protein Restriction
UR - http://www.scopus.com/inward/record.url?scp=84860257864&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860257864&partnerID=8YFLogxK
M3 - Article
C2 - 22201967
AN - SCOPUS:84860257864
SN - 1945-0494
VL - 4 E
SP - 1434
EP - 1450
JO - Frontiers in Bioscience - Elite
JF - Frontiers in Bioscience - Elite
IS - 4
ER -