Mating-type suppression of the DNA-repair defect of the yeast rad6Δ mutation requires the activity of genes in the RAD52 epistasis group

Yu Xin Yan, Louise Prakash, Robert H. Schiestl

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The RAD6 gene of Saccharomyces cerevisiae is required for post-replication repair of UV-damaged DNA, UV mutagenesis, and sporulation. Here, we show that the radiation sensitivity of a MATarad6Δ strain can be suppressed by the MATα2 gene carried on a multicopy plasmid. The a1-α2 suppression is specific to the RAD6 pathway, as mutations in genes required for nucleotide excision repair or for recombinational repair do not show such mating-type suppression. The a1-α2 suppression of the rad6Δ mutation requires the activity of the RAD52 group of genes, suggesting that suppression occurs by channelling of post-replication gaps present in the rad6Δ mutant into the RAD52 recombinational repair pathway. The a1-α2 repressor could mediate this suppression via an enhancement in the expression, or the activity, of recombination genes.

Original languageEnglish (US)
Pages (from-to)12-18
Number of pages7
JournalCurrent Genetics
Volume28
Issue number1
DOIs
StatePublished - Jun 1 1995
Externally publishedYes

Keywords

  • DNA repair
  • Mating type
  • RAD52 gene
  • RAD6 gene
  • UV mutagenesis

ASJC Scopus subject areas

  • Genetics

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