Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of proteins in human cerebrospinal fluid

A. Westman, C. L. Nilsson, R. Ekman

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectra of proteins in cerebrospinal fluid analyzed without prior purification are presented. Less than 100 fmol amounts of proteins in the 10000 to 20000 u mass range and linked to human disease (multiple sclerosis, Alzheimer's disease, and stroke) were detected in a complex mixture of proteins and peptides, in the presence of high concentrations of salts, lipids and free amino acids. The mass resolution was sufficient to distinguish between the non-hydroxylated and hydroxylated forms of a 13 400 u protein. Simple fractionation of the cerebrospinal fluid using microbore-reversed phase high performance liquid chromatography improved signal-to-noise ratios in the mass spectra. High-accuracy peptide mass mapping and database searching were utilized to confirm the identity of several proteins. The presented results show that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry could be used as a tool to perform rapid screening of chemically altered proteins in small volumes of biological fluids.

Original languageEnglish (US)
Pages (from-to)1092-1098
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume12
Issue number16
DOIs
StatePublished - 1998
Externally publishedYes

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Cerebrospinal fluid
Ionization
Mass spectrometry
Desorption
Lasers
Proteins
Peptides
High performance liquid chromatography
Fractionation
Complex Mixtures
Purification
Signal to noise ratio
Screening
Salts
Lipids
Amino Acids
Fluids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of proteins in human cerebrospinal fluid. / Westman, A.; Nilsson, C. L.; Ekman, R.

In: Rapid Communications in Mass Spectrometry, Vol. 12, No. 16, 1998, p. 1092-1098.

Research output: Contribution to journalArticle

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