Matrix control of protein diffusion in biological membranes

D. E. Koppel, Michael Sheetz, M. Schindler

Research output: Contribution to journalArticle

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Abstract

Lateral diffusion coefficients of fluorescently labeled lipid and integral membrane proteins were determined in the membranes of normal and spectrin-deficient spherocytic mouse erythrocytes by the technique of fluorescence redistribution after photobleaching. The results were used to generate a mathematical description of a matrix-control model of membrane protein diffusion. In the spherocytic cells, which lack the principal components of the cytoskeletal matrix of normal cells, the diffusion coefficients of lipid (1.5 ± 0.5 x 10-8 cm2/s) and protein (2.5 ± 0.6 x 10-9 cm2/s) differ only by a factor of 6, close to the difference predicted on the basis of size by the two-dimensional bilayer continuum model of Saffman and Delbruck. In contrast, the membranes of normal cells show a lipid diffusion coefficient (1.4 ± 0.5 x 10-8 cm2/s) that is some 300-fold greater than that of the membrane proteins (4.5 ± 0.8 x 10-11 cm2/s). Analysis of these results, based on the hypothesis that protein diffusion in normal membranes is sterically hindered by a labile matrix, yields an effective matrix surface viscosity consistant with the viscoelastic mechanical properties of the membranes. Thus, a relationship is established between the deformation characteristics of the membrane and the lateral mobility of proteins suspended in the membrane.

Original languageEnglish (US)
Pages (from-to)3576-3580
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number6 I
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

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