Matrix metalloproteinases-9 in preterm and term human parturition

N. Athayde, R. Romero, R. Gomez, E. Maymon, P. Pacora, M. Mazor, B. H. Yoon, S. Fortunato, Ramkumar Menon, F. Ghezzi, S. S. Edwin

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Objective: Spontaneous rupture of the fetal membranes occurs after the commencement of labor in 90% of cases. Recent evidence indicates that the process of parturition requires not only an increase in myometrial contractility and cervical ripening, but also degradation of extracellular matrix in fetal membranes (i.e., leakage of fibronectin into cervico-vaginal secretions). This study was undertaken to determine if parturition is associated with in vivo evidence of increased bioavailability of matrix metalloproteinases-9 (MMP-9) and its inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1). Methods: A cross-sectional study was conducted with women in the following categories: 1) midtrimester (n = 25); 2) preterm labor and intact membranes in the absence of intraamniotic infection (n = 78); 3) term not in labor (n = 25); and 4) term with intact membranes in labor (n = 25). MMP-9, and TIMP-1 were measured using sensitive and specific immunoassays. Results: 1) Spontaneous labor at term was associated with a significant increase in MMP-9 but not in TIMP- 1.2) Women with preterm labor who delivered prematurely had significantly higher concentrations of MMP-9 but not TIMP-1 in amniotic fluid than those with preterm labor who delivered at term. 3) The concentrations of TIMP-1 decreased with advancing gestational age. In contrast, MMP-9 concentrations did not change with advancing gestational age. Conclusions: Spontaneous human parturition is associated with specific changes in the enzymatic machinery responsible for extracellular matrix degradation.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalJournal of Maternal-Fetal Medicine
Volume8
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

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Matrix Metalloproteinase 9
Tissue Inhibitor of Metalloproteinase-1
Parturition
Premature Obstetric Labor
Extraembryonic Membranes
Gestational Age
Extracellular Matrix
Cervical Ripening
Spontaneous Rupture
Matrix Metalloproteinase Inhibitors
Membranes
Second Pregnancy Trimester
Amniotic Fluid
Fibronectins
Immunoassay
Biological Availability
Cross-Sectional Studies
Infection

Keywords

  • Amniotic fluid
  • Matrix metalloproteinases
  • Matrix metalloproteinases inhibitor
  • Parturition
  • Pregnancy
  • Premature labor
  • Prematurity

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pediatrics, Perinatology, and Child Health

Cite this

Matrix metalloproteinases-9 in preterm and term human parturition. / Athayde, N.; Romero, R.; Gomez, R.; Maymon, E.; Pacora, P.; Mazor, M.; Yoon, B. H.; Fortunato, S.; Menon, Ramkumar; Ghezzi, F.; Edwin, S. S.

In: Journal of Maternal-Fetal Medicine, Vol. 8, No. 5, 1999, p. 213-219.

Research output: Contribution to journalArticle

Athayde, N, Romero, R, Gomez, R, Maymon, E, Pacora, P, Mazor, M, Yoon, BH, Fortunato, S, Menon, R, Ghezzi, F & Edwin, SS 1999, 'Matrix metalloproteinases-9 in preterm and term human parturition', Journal of Maternal-Fetal Medicine, vol. 8, no. 5, pp. 213-219. https://doi.org/10.1002/(SICI)1520-6661(199909/10)8:5<213::AID-MFM3>3.0.CO;2-R
Athayde, N. ; Romero, R. ; Gomez, R. ; Maymon, E. ; Pacora, P. ; Mazor, M. ; Yoon, B. H. ; Fortunato, S. ; Menon, Ramkumar ; Ghezzi, F. ; Edwin, S. S. / Matrix metalloproteinases-9 in preterm and term human parturition. In: Journal of Maternal-Fetal Medicine. 1999 ; Vol. 8, No. 5. pp. 213-219.
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abstract = "Objective: Spontaneous rupture of the fetal membranes occurs after the commencement of labor in 90{\%} of cases. Recent evidence indicates that the process of parturition requires not only an increase in myometrial contractility and cervical ripening, but also degradation of extracellular matrix in fetal membranes (i.e., leakage of fibronectin into cervico-vaginal secretions). This study was undertaken to determine if parturition is associated with in vivo evidence of increased bioavailability of matrix metalloproteinases-9 (MMP-9) and its inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1). Methods: A cross-sectional study was conducted with women in the following categories: 1) midtrimester (n = 25); 2) preterm labor and intact membranes in the absence of intraamniotic infection (n = 78); 3) term not in labor (n = 25); and 4) term with intact membranes in labor (n = 25). MMP-9, and TIMP-1 were measured using sensitive and specific immunoassays. Results: 1) Spontaneous labor at term was associated with a significant increase in MMP-9 but not in TIMP- 1.2) Women with preterm labor who delivered prematurely had significantly higher concentrations of MMP-9 but not TIMP-1 in amniotic fluid than those with preterm labor who delivered at term. 3) The concentrations of TIMP-1 decreased with advancing gestational age. In contrast, MMP-9 concentrations did not change with advancing gestational age. Conclusions: Spontaneous human parturition is associated with specific changes in the enzymatic machinery responsible for extracellular matrix degradation.",
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T1 - Matrix metalloproteinases-9 in preterm and term human parturition

AU - Athayde, N.

AU - Romero, R.

AU - Gomez, R.

AU - Maymon, E.

AU - Pacora, P.

AU - Mazor, M.

AU - Yoon, B. H.

AU - Fortunato, S.

AU - Menon, Ramkumar

AU - Ghezzi, F.

AU - Edwin, S. S.

PY - 1999

Y1 - 1999

N2 - Objective: Spontaneous rupture of the fetal membranes occurs after the commencement of labor in 90% of cases. Recent evidence indicates that the process of parturition requires not only an increase in myometrial contractility and cervical ripening, but also degradation of extracellular matrix in fetal membranes (i.e., leakage of fibronectin into cervico-vaginal secretions). This study was undertaken to determine if parturition is associated with in vivo evidence of increased bioavailability of matrix metalloproteinases-9 (MMP-9) and its inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1). Methods: A cross-sectional study was conducted with women in the following categories: 1) midtrimester (n = 25); 2) preterm labor and intact membranes in the absence of intraamniotic infection (n = 78); 3) term not in labor (n = 25); and 4) term with intact membranes in labor (n = 25). MMP-9, and TIMP-1 were measured using sensitive and specific immunoassays. Results: 1) Spontaneous labor at term was associated with a significant increase in MMP-9 but not in TIMP- 1.2) Women with preterm labor who delivered prematurely had significantly higher concentrations of MMP-9 but not TIMP-1 in amniotic fluid than those with preterm labor who delivered at term. 3) The concentrations of TIMP-1 decreased with advancing gestational age. In contrast, MMP-9 concentrations did not change with advancing gestational age. Conclusions: Spontaneous human parturition is associated with specific changes in the enzymatic machinery responsible for extracellular matrix degradation.

AB - Objective: Spontaneous rupture of the fetal membranes occurs after the commencement of labor in 90% of cases. Recent evidence indicates that the process of parturition requires not only an increase in myometrial contractility and cervical ripening, but also degradation of extracellular matrix in fetal membranes (i.e., leakage of fibronectin into cervico-vaginal secretions). This study was undertaken to determine if parturition is associated with in vivo evidence of increased bioavailability of matrix metalloproteinases-9 (MMP-9) and its inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1). Methods: A cross-sectional study was conducted with women in the following categories: 1) midtrimester (n = 25); 2) preterm labor and intact membranes in the absence of intraamniotic infection (n = 78); 3) term not in labor (n = 25); and 4) term with intact membranes in labor (n = 25). MMP-9, and TIMP-1 were measured using sensitive and specific immunoassays. Results: 1) Spontaneous labor at term was associated with a significant increase in MMP-9 but not in TIMP- 1.2) Women with preterm labor who delivered prematurely had significantly higher concentrations of MMP-9 but not TIMP-1 in amniotic fluid than those with preterm labor who delivered at term. 3) The concentrations of TIMP-1 decreased with advancing gestational age. In contrast, MMP-9 concentrations did not change with advancing gestational age. Conclusions: Spontaneous human parturition is associated with specific changes in the enzymatic machinery responsible for extracellular matrix degradation.

KW - Amniotic fluid

KW - Matrix metalloproteinases

KW - Matrix metalloproteinases inhibitor

KW - Parturition

KW - Pregnancy

KW - Premature labor

KW - Prematurity

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