Matrix metalloproteinases and their tissue inhibitors in severely burned children

Mohan R K Dasu, Marcus Spies, Robert E. Barrow, David Herndon

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Severe burns cause not only skin injury but several marked systemic derangements. During wound healing, matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases play an important role in tissue regeneration and remodeling processes. Therefore, in the present study, we determined the serum levels of MMPs and tissue inhibitor of metalloproteinase-1 in burn patients over time. Serum samples from 12 severely burned children (mean age 7.9 ± 2.5 years) with >40% total body surface area burns were obtained within 0.5 hours, 3, 7, and 21 days after injury. Pro-MMP-1, MMP-3, MMP-9, and tissue inhibitor of metalloproteinase-1 serum levels were assayed by enzyme-linked immunoassay and compared to normal healthy volunteers. Two-way analysis of variance and Bonferroni's test were used for statistical analysis. Pro-MMP-1 levels in the serum were significantly elevated by the seventh day after burn. MMP-3 and MMP-9 levels showed significant increases by day 3 and 21 compared to normals, respectively. Tissue inhibitor of metalloproteinase-1 levels did not change with time after burn but were significantly higher by 3 days after burn compared to normal serum. In conclusion, changes in MMPs and tissue inhibitor of metalloproteinase-1 occur in burn patients and those changes may be a mechanism beneficial to wound healing.

Original languageEnglish (US)
Pages (from-to)177-180
Number of pages4
JournalWound Repair and Regeneration
Volume11
Issue number3
DOIs
StatePublished - May 2003
Externally publishedYes

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Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 3
Serum
Matrix Metalloproteinase 1
Matrix Metalloproteinase 9
Burns
Wound Healing
Healthy Volunteers
Tissue Inhibitor of Metalloproteinases
Body Surface Area
Wounds and Injuries
Immunoenzyme Techniques
Regeneration
Analysis of Variance
Skin

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Matrix metalloproteinases and their tissue inhibitors in severely burned children. / Dasu, Mohan R K; Spies, Marcus; Barrow, Robert E.; Herndon, David.

In: Wound Repair and Regeneration, Vol. 11, No. 3, 05.2003, p. 177-180.

Research output: Contribution to journalArticle

Dasu, Mohan R K ; Spies, Marcus ; Barrow, Robert E. ; Herndon, David. / Matrix metalloproteinases and their tissue inhibitors in severely burned children. In: Wound Repair and Regeneration. 2003 ; Vol. 11, No. 3. pp. 177-180.
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