Measurement of dynorphins with 3H-etorphine and canine limbic system receptors

Danxia Liu, Dominic M. Desiderio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A canine limbic system preparation was used as the source of opioid peptide receptors to screen biologic extracts for the presence of opioid receptoractive peptides following their gradient RP-HPLC separation. Eight synthetic dynorphin peptides were studied for their ability to displace the commonly-used ligand 3H-etorphine from the canine limbic system P2 preparation. The peptides studied included the dynorphins 1-7, 1-8, 1-9, 1-10, 1-12, 1-13, 1-17, and dynorphin B. Two different types of opioid peptide molecules were utilized for the determination of the level of non-specific binding. In one study, methionine enkephalin, and in the second study each one of the eight corresponding dynorphins, was used for determination of non-specific binding. The experimental data indicated that 3H-etorphine bound to the canine limbic system P2 receptors, and that those dynorphins displaced effectively the 3H-etorphine from those receptors.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalNeuropeptides
Volume12
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Etorphine
Dynorphins
Limbic System
Opioid Peptides
Canidae
Methionine Enkephalin
Peptides
Peptide Receptors
Opioid Receptors
High Pressure Liquid Chromatography
Ligands
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Clinical Neurology
  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

Measurement of dynorphins with 3H-etorphine and canine limbic system receptors. / Liu, Danxia; Desiderio, Dominic M.

In: Neuropeptides, Vol. 12, No. 1, 1988, p. 35-40.

Research output: Contribution to journalArticle

Liu, Danxia ; Desiderio, Dominic M. / Measurement of dynorphins with 3H-etorphine and canine limbic system receptors. In: Neuropeptides. 1988 ; Vol. 12, No. 1. pp. 35-40.
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