TY - JOUR
T1 - Measurement of dynorphins with 3H-etorphine and canine limbic system receptors
AU - Liu, Danxia
AU - Desiderio, Dominic M.
N1 - Funding Information:
The authorsg ratefullya cknowledget he financial assistance from the NationalI nstituteso f Health (GM26666),t he typing assistancoef L. Rutherford;a ndt he assistancoe fD . Cubbinsi n preparingt he microcomputedr atap lots (Figures1 and 2).
PY - 1988/7
Y1 - 1988/7
N2 - A canine limbic system preparation was used as the source of opioid peptide receptors to screen biologic extracts for the presence of opioid receptoractive peptides following their gradient RP-HPLC separation. Eight synthetic dynorphin peptides were studied for their ability to displace the commonly-used ligand 3H-etorphine from the canine limbic system P2 preparation. The peptides studied included the dynorphins 1-7, 1-8, 1-9, 1-10, 1-12, 1-13, 1-17, and dynorphin B. Two different types of opioid peptide molecules were utilized for the determination of the level of non-specific binding. In one study, methionine enkephalin, and in the second study each one of the eight corresponding dynorphins, was used for determination of non-specific binding. The experimental data indicated that 3H-etorphine bound to the canine limbic system P2 receptors, and that those dynorphins displaced effectively the 3H-etorphine from those receptors.
AB - A canine limbic system preparation was used as the source of opioid peptide receptors to screen biologic extracts for the presence of opioid receptoractive peptides following their gradient RP-HPLC separation. Eight synthetic dynorphin peptides were studied for their ability to displace the commonly-used ligand 3H-etorphine from the canine limbic system P2 preparation. The peptides studied included the dynorphins 1-7, 1-8, 1-9, 1-10, 1-12, 1-13, 1-17, and dynorphin B. Two different types of opioid peptide molecules were utilized for the determination of the level of non-specific binding. In one study, methionine enkephalin, and in the second study each one of the eight corresponding dynorphins, was used for determination of non-specific binding. The experimental data indicated that 3H-etorphine bound to the canine limbic system P2 receptors, and that those dynorphins displaced effectively the 3H-etorphine from those receptors.
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U2 - 10.1016/S0143-4179(98)90007-5
DO - 10.1016/S0143-4179(98)90007-5
M3 - Article
C2 - 2901686
AN - SCOPUS:0023819751
SN - 0143-4179
VL - 12
SP - 35
EP - 40
JO - Neuropeptides
JF - Neuropeptides
IS - 1
ER -