Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection

Hui Tang, Bing Tian, Allan R. Brasier, Lawrence Sowers, Kangling Zhang

Research output: Contribution to journalArticle

3 Scopus citations


Accumulating evidence suggests that cellular metabolites and nutrition levels control epigenetic modifications, including histone methylation. However, it is not currently possible to measure the metabolic control of histone methylation. Here we report a novel detection method to monitor methyl transfer from serine to histones through the one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation signature ions generated in high-energy-dissociation (HCD) tandem mass spectrometry. This method is a long-needed tool to study the metabolic control of histone methylation.

Original languageEnglish (US)
Article number31537
JournalScientific Reports
StatePublished - Aug 17 2016


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