Measurement of protein fractional synthesis and breakdown rates in the skin of rabbits using a subflooding dose method

Xiao jun Zhang, David L. Chinkes, David Herndon, Robert R. Wolfe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The flooding dose method continues to be useful in measuring protein fractional synthetic rate (FSR) in a tissue. However, flooding of free amino acid pools eliminates enrichment difference between plasma and tissue free amino acid pools, which makes it impossible to concomitantly measure protein fractional breakdown rate (FBR). We hypothesized that a subflooding dose of an amino acid reduces the enrichment difference between plasma and tissue free amino acid pool to a minimal measurable level, thus allowing concomitant measurement of protein FSR and FBR. Phenylalanine (40% enriched) at 50 mg/kg was intravenously injected as a bolus in 6 anesthetized rabbits. Arterial blood and chest skin samples were taken before the injection and for 120 minutes after the injection. Fractional breakdown rate of skin protein calculated from 15-60-120-minute sampling times was 11.3%/d ± 2.0%/d, which was close (P = .66) to the corresponding FSR of 10.0%/d ± 2.3%/d. The subflooding dose injection did not disturb the FBR approach because modifications on the FBR equation to account for the changes of plasma phenylalanine concentration resulted in the same value (11.5%/d ± 1.4%/d). The FBR was positively correlated with the FSR (r = 0.80, P < .05). These findings indicate maintenance of protein mass in the skin, which is a metabolic characteristic of the skin. This subflooding dose method provides a methodological choice to concomitantly measure both FSR and FBR in a tissue.

Original languageEnglish (US)
Pages (from-to)1239-1247
Number of pages9
JournalMetabolism: Clinical and Experimental
Volume58
Issue number9
DOIs
StatePublished - Sep 2009

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Rabbits
Skin
Amino Acids
Proteins
Phenylalanine
Injections
Thorax
Maintenance

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Measurement of protein fractional synthesis and breakdown rates in the skin of rabbits using a subflooding dose method. / Zhang, Xiao jun; Chinkes, David L.; Herndon, David; Wolfe, Robert R.

In: Metabolism: Clinical and Experimental, Vol. 58, No. 9, 09.2009, p. 1239-1247.

Research output: Contribution to journalArticle

Zhang, Xiao jun ; Chinkes, David L. ; Herndon, David ; Wolfe, Robert R. / Measurement of protein fractional synthesis and breakdown rates in the skin of rabbits using a subflooding dose method. In: Metabolism: Clinical and Experimental. 2009 ; Vol. 58, No. 9. pp. 1239-1247.
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