Measurement of skin protein breakdown in a rat model

Elena Volpi, Marc G. Jeschke, David Herndon, Robert R. Wolfe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Whereas skin protein synthesis can be measured with different approaches, no method potentially applicable in humans is available for measurement of skin protein breakdown. To that end, we measured mixed skin fractional protein breakdown (FBR) in a rat model by use of a stable isotope method (tracee release method) originally developed to measure muscle protein breakdown. Skin mixed protein and collagen fractional synthesis rates (FSR) were also measured. A primed continuous infusion of L-[ring- 2H 5]phenylalanine and α-[5,5,5- 2H 3]ketoisocaproate (KIC) was given for 6 h. Arterial and skin phenylalanine and leucine free enrichments were measured at plateau (5-6 h) and during the decay that followed after the infusion was stopped. Skin FBR (%/h) was 0.260 ± 0.011 with phenylalanine and 0.201 ± 0.032 with KIC/leucine [P = not significant (NS)]. Mixed skin FSR (%/h) was 0.169 ± 0.055 with phenylalanine and 0.146 ± 0.020 with KIC/leucine (P = NS). Collagen FSR was 0.124 ± 0.023%/h (P = NS vs. mixed protein FSR). The tracee release method is a sensitive method for measurement of skin protein breakdown; however, given the high intersubject variability of FSR, the calculation of skin net balance is not advisable.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume279
Issue number4 42-4
StatePublished - 2000

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Rats
Skin
Phenylalanine
Proteins
Leucine
Collagen
Muscle Proteins
Isotopes

Keywords

  • Collagen
  • Protein synthesis
  • Proteolysis

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Measurement of skin protein breakdown in a rat model. / Volpi, Elena; Jeschke, Marc G.; Herndon, David; Wolfe, Robert R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 279, No. 4 42-4, 2000.

Research output: Contribution to journalArticle

Volpi, Elena ; Jeschke, Marc G. ; Herndon, David ; Wolfe, Robert R. / Measurement of skin protein breakdown in a rat model. In: American Journal of Physiology - Endocrinology and Metabolism. 2000 ; Vol. 279, No. 4 42-4.
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