Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila

Ashok Chopra, Tuyet N. Vo, Clifford W. Houston

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalFEMS Microbiology Letters
Volume91
Issue number1
DOIs
StatePublished - Feb 1 1992

Fingerprint

Aeromonas hydrophila
Enterotoxins
Cricetulus
Ovary
Cholera Toxin
Fishes
Monoclonal Antibodies

Keywords

  • Aerolysin
  • Aeromonas hydrophila
  • cAMP
  • Cytotonic enterotoxin
  • Neutralization
  • PgE

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila. / Chopra, Ashok; Vo, Tuyet N.; Houston, Clifford W.

In: FEMS Microbiology Letters, Vol. 91, No. 1, 01.02.1992, p. 15-19.

Research output: Contribution to journalArticle

Chopra, Ashok ; Vo, Tuyet N. ; Houston, Clifford W. / Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila. In: FEMS Microbiology Letters. 1992 ; Vol. 91, No. 1. pp. 15-19.
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