Abstract
In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 15-19 |
| Number of pages | 5 |
| Journal | FEMS Microbiology Letters |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 1992 |
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Keywords
- Aerolysin
- Aeromonas hydrophila
- cAMP
- Cytotonic enterotoxin
- Neutralization
- PgE
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila. / Chopra, Ashok; Vo, Tuyet N.; Houston, Clifford W.
In: FEMS Microbiology Letters, Vol. 91, No. 1, 01.02.1992, p. 15-19.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila
AU - Chopra, Ashok
AU - Vo, Tuyet N.
AU - Houston, Clifford W.
PY - 1992/2/1
Y1 - 1992/2/1
N2 - In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.
AB - In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.
KW - Aerolysin
KW - Aeromonas hydrophila
KW - cAMP
KW - Cytotonic enterotoxin
KW - Neutralization
KW - PgE
UR - http://www.scopus.com/inward/record.url?scp=0026816371&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026816371&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(92)90556-4
DO - 10.1016/0378-1097(92)90556-4
M3 - Article
VL - 91
SP - 15
EP - 19
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -