Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila

Ashok K. Chopra, Tuyet N. Vo, Clifford W. Houston

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalFEMS Microbiology Letters
Volume91
Issue number1
DOIs
StatePublished - Feb 1 1992

Keywords

  • Aerolysin
  • Aeromonas hydrophila
  • Cytotonic enterotoxin
  • Neutralization
  • PgE
  • cAMP

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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