Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila

Ashok K. Chopra; Tuyet N. Vo; Clifford Houston

doi:10.1016/0378-1097(92)90556-4

Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila

Ashok K. Chopra, Tuyet N. Vo, Clifford Houston

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE₂ levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE₂ levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

Original language	English (US)
Pages (from-to)	15-19
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(92)90556-4
State	Published - Feb 1 1992

Keywords

Aerolysin
Aeromonas hydrophila
Cytotonic enterotoxin
Neutralization
PgE
cAMP

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/0378-1097(92)90556-4

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title = "Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila",

abstract = "In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.",

keywords = "Aerolysin, Aeromonas hydrophila, Cytotonic enterotoxin, Neutralization, PgE, cAMP",

author = "Chopra, {Ashok K.} and Vo, {Tuyet N.} and Clifford Houston",

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AU - Chopra, Ashok K.

AU - Vo, Tuyet N.

AU - Houston, Clifford

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N2 - In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

AB - In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

KW - Aerolysin

KW - Aeromonas hydrophila

KW - Cytotonic enterotoxin

KW - Neutralization

KW - PgE

KW - cAMP

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Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila

Abstract

Keywords

ASJC Scopus subject areas

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