Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia

G. Redl, L. Woodson, L. D. Traber, C. S. Rogers, S. Abdi, J. T. Flynn, David Herndon, D. L. Traber

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have previously reported an increase in plasma levels of atrial natriuretic factor (ANF) in an ovine model of endotoxemia. The purpose of this study was to determine if this IR-ANF release was mediated by the increase of right atrial pressure (RAP) and right heart volumes concomitantly observed following endotoxin (LPS) administration. We studied right ventricular function, renal blood flow (RBF), urinary output (UO), urinary clearance of free water (C(H20)), urinary osmolality (U(OSM)), sodium excretion (UE(NA)), and the plasma IR-ANF concentration (radioimmunoassay), following the administration of an E. coli LPS bolus (1 μg/kg) with (group O, n = 8) and without (group E, n = 10) pretreatment with OKY-046, a selective thromboxane synthetase inhibitor. LPS induced early increases in RAP, right ventricular end-systolic (RVESV) and end-diastolic (RVEDV) volumes, heart rate (HR), and IR-ANF, and delayed increases in RBF, UO, and C(H20). OKY-046 prevented the elevation of RAP, RVEDV, and RVESV; however, both groups showed virtually identical increases in IR-ANF (E:20.03 ± 3.8 to 192.33 ± 35.47 pg/ml, O: 17.9 ± 4.1 to 159.5 ± 23 pg/ml) as well as an increase of HR, RBF, UO, and C(H20). The increase in IR-ANF release noted following the administration of LPS in an ovine model does not appear to be related to the early elevations in right heart volumes or atrial distension.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalCirculatory Shock
Volume38
Issue number1
StatePublished - 1992
Externally publishedYes

Fingerprint

Endotoxemia
Atrial Natriuretic Factor
Sheep
Atrial Pressure
Renal Circulation
Cardiac Volume
Heart Rate
Thromboxane-A Synthase
Right Ventricular Function
Endotoxins
Osmolar Concentration
Radioimmunoassay
Sodium
Escherichia coli
Water

Keywords

  • atrial natriuretic peptide
  • right heart
  • sepsis
  • sheep
  • thermodilution

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Redl, G., Woodson, L., Traber, L. D., Rogers, C. S., Abdi, S., Flynn, J. T., ... Traber, D. L. (1992). Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia. Circulatory Shock, 38(1), 34-41.

Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia. / Redl, G.; Woodson, L.; Traber, L. D.; Rogers, C. S.; Abdi, S.; Flynn, J. T.; Herndon, David; Traber, D. L.

In: Circulatory Shock, Vol. 38, No. 1, 1992, p. 34-41.

Research output: Contribution to journalArticle

Redl, G, Woodson, L, Traber, LD, Rogers, CS, Abdi, S, Flynn, JT, Herndon, D & Traber, DL 1992, 'Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia', Circulatory Shock, vol. 38, no. 1, pp. 34-41.
Redl G, Woodson L, Traber LD, Rogers CS, Abdi S, Flynn JT et al. Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia. Circulatory Shock. 1992;38(1):34-41.
Redl, G. ; Woodson, L. ; Traber, L. D. ; Rogers, C. S. ; Abdi, S. ; Flynn, J. T. ; Herndon, David ; Traber, D. L. / Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia. In: Circulatory Shock. 1992 ; Vol. 38, No. 1. pp. 34-41.
@article{f778f5f8e754464cb3c47d45b9fe1a27,
title = "Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia",
abstract = "We have previously reported an increase in plasma levels of atrial natriuretic factor (ANF) in an ovine model of endotoxemia. The purpose of this study was to determine if this IR-ANF release was mediated by the increase of right atrial pressure (RAP) and right heart volumes concomitantly observed following endotoxin (LPS) administration. We studied right ventricular function, renal blood flow (RBF), urinary output (UO), urinary clearance of free water (C(H20)), urinary osmolality (U(OSM)), sodium excretion (UE(NA)), and the plasma IR-ANF concentration (radioimmunoassay), following the administration of an E. coli LPS bolus (1 μg/kg) with (group O, n = 8) and without (group E, n = 10) pretreatment with OKY-046, a selective thromboxane synthetase inhibitor. LPS induced early increases in RAP, right ventricular end-systolic (RVESV) and end-diastolic (RVEDV) volumes, heart rate (HR), and IR-ANF, and delayed increases in RBF, UO, and C(H20). OKY-046 prevented the elevation of RAP, RVEDV, and RVESV; however, both groups showed virtually identical increases in IR-ANF (E:20.03 ± 3.8 to 192.33 ± 35.47 pg/ml, O: 17.9 ± 4.1 to 159.5 ± 23 pg/ml) as well as an increase of HR, RBF, UO, and C(H20). The increase in IR-ANF release noted following the administration of LPS in an ovine model does not appear to be related to the early elevations in right heart volumes or atrial distension.",
keywords = "atrial natriuretic peptide, right heart, sepsis, sheep, thermodilution",
author = "G. Redl and L. Woodson and Traber, {L. D.} and Rogers, {C. S.} and S. Abdi and Flynn, {J. T.} and David Herndon and Traber, {D. L.}",
year = "1992",
language = "English (US)",
volume = "38",
pages = "34--41",
journal = "Journal of inflammation",
issn = "1078-7852",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Mechanism of immunoreactive atrial natriuretic factor release in an ovine model of endotoxemia

AU - Redl, G.

AU - Woodson, L.

AU - Traber, L. D.

AU - Rogers, C. S.

AU - Abdi, S.

AU - Flynn, J. T.

AU - Herndon, David

AU - Traber, D. L.

PY - 1992

Y1 - 1992

N2 - We have previously reported an increase in plasma levels of atrial natriuretic factor (ANF) in an ovine model of endotoxemia. The purpose of this study was to determine if this IR-ANF release was mediated by the increase of right atrial pressure (RAP) and right heart volumes concomitantly observed following endotoxin (LPS) administration. We studied right ventricular function, renal blood flow (RBF), urinary output (UO), urinary clearance of free water (C(H20)), urinary osmolality (U(OSM)), sodium excretion (UE(NA)), and the plasma IR-ANF concentration (radioimmunoassay), following the administration of an E. coli LPS bolus (1 μg/kg) with (group O, n = 8) and without (group E, n = 10) pretreatment with OKY-046, a selective thromboxane synthetase inhibitor. LPS induced early increases in RAP, right ventricular end-systolic (RVESV) and end-diastolic (RVEDV) volumes, heart rate (HR), and IR-ANF, and delayed increases in RBF, UO, and C(H20). OKY-046 prevented the elevation of RAP, RVEDV, and RVESV; however, both groups showed virtually identical increases in IR-ANF (E:20.03 ± 3.8 to 192.33 ± 35.47 pg/ml, O: 17.9 ± 4.1 to 159.5 ± 23 pg/ml) as well as an increase of HR, RBF, UO, and C(H20). The increase in IR-ANF release noted following the administration of LPS in an ovine model does not appear to be related to the early elevations in right heart volumes or atrial distension.

AB - We have previously reported an increase in plasma levels of atrial natriuretic factor (ANF) in an ovine model of endotoxemia. The purpose of this study was to determine if this IR-ANF release was mediated by the increase of right atrial pressure (RAP) and right heart volumes concomitantly observed following endotoxin (LPS) administration. We studied right ventricular function, renal blood flow (RBF), urinary output (UO), urinary clearance of free water (C(H20)), urinary osmolality (U(OSM)), sodium excretion (UE(NA)), and the plasma IR-ANF concentration (radioimmunoassay), following the administration of an E. coli LPS bolus (1 μg/kg) with (group O, n = 8) and without (group E, n = 10) pretreatment with OKY-046, a selective thromboxane synthetase inhibitor. LPS induced early increases in RAP, right ventricular end-systolic (RVESV) and end-diastolic (RVEDV) volumes, heart rate (HR), and IR-ANF, and delayed increases in RBF, UO, and C(H20). OKY-046 prevented the elevation of RAP, RVEDV, and RVESV; however, both groups showed virtually identical increases in IR-ANF (E:20.03 ± 3.8 to 192.33 ± 35.47 pg/ml, O: 17.9 ± 4.1 to 159.5 ± 23 pg/ml) as well as an increase of HR, RBF, UO, and C(H20). The increase in IR-ANF release noted following the administration of LPS in an ovine model does not appear to be related to the early elevations in right heart volumes or atrial distension.

KW - atrial natriuretic peptide

KW - right heart

KW - sepsis

KW - sheep

KW - thermodilution

UR - http://www.scopus.com/inward/record.url?scp=0026706380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026706380&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 34

EP - 41

JO - Journal of inflammation

JF - Journal of inflammation

SN - 1078-7852

IS - 1

ER -