Mechanism of intracellular cAMP sensor Epac2 activation: cAMP-induced conformational changes identified by amide hydrogen/Deuterium Exchange Mass Spectrometry (DXMS)

Keyphrases

• Allosteric Modulator 14%
• Amides 100%
• C-terminus 14%
• Catalytic Core 14%
• Cell Targeting 14%
• Conformational Change 100%
• Conformational Dynamics 14%
• Core Making Process 14%
• Effector Binding 14%
• Epac 14%
• Epac2 100%
• Guanine nucleotide Exchange Factor 14%
• Hairpin Structure 14%
• Highly Dynamic 14%
• Hinge Motion 14%
• Hydrogen-deuterium Exchange Mass Spectrometry 100%
• In Vivo Function 14%
• Intracellular cAMP 100%
• Intracellular Signaling 14%
• Ligand Binding Site 14%
• Ligand-binding Domain 42%
• Peptide Amide 14%
• Pleckstrin 14%
• Rap1 28%
• Regulatory Component 14%
• Second Messenger 14%
• Steric Hindrance 14%
• Structural Dynamics 14%
• Structural Motif 14%

Biochemistry, Genetics and Molecular Biology

• Binding Domain 25%
• Binding Site 8%
• C-Terminus 8%
• Conformational Change 100%
• Crystal Structure 16%
• Cyclic Adenosine Monophosphate 100%
• Dishevelled 8%
• Dynamics 8%
• Guanine Nucleotide Exchange Factor 8%
• Hydrogen/Deuterium Exchange Mass Spectrometry 100%
• Ligand Binding 8%
• Motion 8%
• Pleckstrin 8%
• Second Messenger System 8%
• Solution and Solubility 8%
• Stereospecificity 8%
• Structural Motif 8%