Mechanisms controlling cell size and shape during isotropic cell spreading

Yuguang Xiong, Padmini Rangamani, Marc Antoine Fardin, Azi Lipshtat, Benjamin Dubin-Thaler, Olivier Rossier, Michael Sheetz, Ravi Iyengar

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cell motility is important for many developmental and physiological processes. Motility arises from interactions between physical forces at the cell surface membrane and the biochemical reactions that control the actin cytoskeleton. To computationally analyze how these factors interact, we built a three-dimensional stochastic model of the experimentally observed isotropic spreading phase of mammalian fibroblasts. The multiscale model is composed at the microscopic levels of three actin filament remodeling reactions that occur stochastically in space and time, and these reactions are regulated by the membrane forces due to membrane surface resistance (load) and bending energy. The macroscopic output of the model (isotropic spreading of the whole cell) occurs due to the movement of the leading edge, resulting solely from membrane force-constrained biochemical reactions. Numerical simulations indicate that our model qualitatively captures the experimentally observed isotropic cell-spreading behavior. The model predicts that increasing the capping protein concentration will lead to a proportional decrease in the spread radius of the cell. This prediction was experimentally confirmed with the use of Cytochalasin D, which caps growing actin filaments. Similarly, the predicted effect of actin monomer concentration was experimentally verified by using Latrunculin A. Parameter variation analyses indicate that membrane physical forces control cell shape during spreading, whereas the biochemical reactions underlying actin cytoskeleton dynamics control cell size (i.e., the rate of spreading). Thus, during cell spreading, a balance between the biochemical and biophysical properties determines the cell size and shape. These mechanistic insights can provide a format for understanding how force and chemical signals together modulate cellular regulatory networks to control cell motility.

Original languageEnglish (US)
Pages (from-to)2136-2146
Number of pages11
JournalBiophysical Journal
Volume98
Issue number10
DOIs
StatePublished - May 19 2010
Externally publishedYes

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Cell Shape
Actin Cytoskeleton
Cell Size
Membranes
Cell Movement
Physiological Phenomena
Cytochalasin D
Statistical Factor Analysis
Actins
Fibroblasts
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Xiong, Y., Rangamani, P., Fardin, M. A., Lipshtat, A., Dubin-Thaler, B., Rossier, O., ... Iyengar, R. (2010). Mechanisms controlling cell size and shape during isotropic cell spreading. Biophysical Journal, 98(10), 2136-2146. https://doi.org/10.1016/j.bpj.2010.01.059

Mechanisms controlling cell size and shape during isotropic cell spreading. / Xiong, Yuguang; Rangamani, Padmini; Fardin, Marc Antoine; Lipshtat, Azi; Dubin-Thaler, Benjamin; Rossier, Olivier; Sheetz, Michael; Iyengar, Ravi.

In: Biophysical Journal, Vol. 98, No. 10, 19.05.2010, p. 2136-2146.

Research output: Contribution to journalArticle

Xiong, Y, Rangamani, P, Fardin, MA, Lipshtat, A, Dubin-Thaler, B, Rossier, O, Sheetz, M & Iyengar, R 2010, 'Mechanisms controlling cell size and shape during isotropic cell spreading', Biophysical Journal, vol. 98, no. 10, pp. 2136-2146. https://doi.org/10.1016/j.bpj.2010.01.059
Xiong Y, Rangamani P, Fardin MA, Lipshtat A, Dubin-Thaler B, Rossier O et al. Mechanisms controlling cell size and shape during isotropic cell spreading. Biophysical Journal. 2010 May 19;98(10):2136-2146. https://doi.org/10.1016/j.bpj.2010.01.059
Xiong, Yuguang ; Rangamani, Padmini ; Fardin, Marc Antoine ; Lipshtat, Azi ; Dubin-Thaler, Benjamin ; Rossier, Olivier ; Sheetz, Michael ; Iyengar, Ravi. / Mechanisms controlling cell size and shape during isotropic cell spreading. In: Biophysical Journal. 2010 ; Vol. 98, No. 10. pp. 2136-2146.
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