Melatonin is a scavenger of peroxynitrite

Eli Gilad; Salvatore Cuzzocrea; Basilia Zingarelli; Andrew L. Salzman; Csaba Szabó

doi:10.1016/S0024-3205(97)00008-8

Melatonin is a scavenger of peroxynitrite

Eli Gilad, Salvatore Cuzzocrea, Basilia Zingarelli, Andrew L. Salzman, Csaba Szabó

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

Peroxynitrite is a toxic oxidant formed from the reaction of superoxide and nitric oxide under conditions of inflammation and oxidant stress. Here we demonstrate that the pineal neurohormone melatonin inhibits peroxynitrite-mediated oxidant processes. Melatonin caused a dose-dependent inhibition of the oxidation of dihydrorhodamine 123 to rhodamine in vitro. Moreover, in cultured J774 macrophages, melatonin inhibited the development of DNA single strand breakage in response to peroxynitrite and reduced the suppression of mitochondrial respiration. Thus, melatonin appears to be a scavenger of peroxynitrite. This action may contribute to the antioxidant and antiinflammatory effects of melatonin in various pathophysiological conditions.

Original language	English (US)
Pages (from-to)	PL169-PL174
Journal	Life Sciences
Volume	60
Issue number	10
DOIs	https://doi.org/10.1016/S0024-3205(97)00008-8
State	Published - Jan 31 1997

Keywords

Free radicals
Melatonin
Nitric oxide
Pineal
Shock

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/S0024-3205(97)00008-8

Cite this

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title = "Melatonin is a scavenger of peroxynitrite",

abstract = "Peroxynitrite is a toxic oxidant formed from the reaction of superoxide and nitric oxide under conditions of inflammation and oxidant stress. Here we demonstrate that the pineal neurohormone melatonin inhibits peroxynitrite-mediated oxidant processes. Melatonin caused a dose-dependent inhibition of the oxidation of dihydrorhodamine 123 to rhodamine in vitro. Moreover, in cultured J774 macrophages, melatonin inhibited the development of DNA single strand breakage in response to peroxynitrite and reduced the suppression of mitochondrial respiration. Thus, melatonin appears to be a scavenger of peroxynitrite. This action may contribute to the antioxidant and antiinflammatory effects of melatonin in various pathophysiological conditions.",

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author = "Eli Gilad and Salvatore Cuzzocrea and Basilia Zingarelli and Salzman, {Andrew L.} and Csaba Szab{\'o}",

note = "Funding Information: This work was supported, in part, by a Grant from the American Lung Association (RG033N) to Dr. Szabo.",

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T1 - Melatonin is a scavenger of peroxynitrite

AU - Gilad, Eli

AU - Cuzzocrea, Salvatore

AU - Zingarelli, Basilia

AU - Salzman, Andrew L.

AU - Szabó, Csaba

N1 - Funding Information: This work was supported, in part, by a Grant from the American Lung Association (RG033N) to Dr. Szabo.

PY - 1997/1/31

Y1 - 1997/1/31

N2 - Peroxynitrite is a toxic oxidant formed from the reaction of superoxide and nitric oxide under conditions of inflammation and oxidant stress. Here we demonstrate that the pineal neurohormone melatonin inhibits peroxynitrite-mediated oxidant processes. Melatonin caused a dose-dependent inhibition of the oxidation of dihydrorhodamine 123 to rhodamine in vitro. Moreover, in cultured J774 macrophages, melatonin inhibited the development of DNA single strand breakage in response to peroxynitrite and reduced the suppression of mitochondrial respiration. Thus, melatonin appears to be a scavenger of peroxynitrite. This action may contribute to the antioxidant and antiinflammatory effects of melatonin in various pathophysiological conditions.

AB - Peroxynitrite is a toxic oxidant formed from the reaction of superoxide and nitric oxide under conditions of inflammation and oxidant stress. Here we demonstrate that the pineal neurohormone melatonin inhibits peroxynitrite-mediated oxidant processes. Melatonin caused a dose-dependent inhibition of the oxidation of dihydrorhodamine 123 to rhodamine in vitro. Moreover, in cultured J774 macrophages, melatonin inhibited the development of DNA single strand breakage in response to peroxynitrite and reduced the suppression of mitochondrial respiration. Thus, melatonin appears to be a scavenger of peroxynitrite. This action may contribute to the antioxidant and antiinflammatory effects of melatonin in various pathophysiological conditions.

KW - Free radicals

KW - Melatonin

KW - Nitric oxide

KW - Pineal

KW - Shock

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SP - PL169-PL174

JO - Life Sciences

JF - Life Sciences

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ER -

Melatonin is a scavenger of peroxynitrite

Abstract

Keywords

ASJC Scopus subject areas

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