Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells

Tingting Jiang, Qing Chang, Zhenyang Zhao, Saimei Yan, Ling Wang, Jiyang Cai, Gezhi Xu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Oxidative stress is a contributing factor to the development and progression of diabetic retinopathy, a leading cause of blindness in people at working age worldwide. Recent studies showed that Müller cells play key roles in diabetic retinopathy and produce vascular endothelial growth factor (VEGF) that regulates retinal vascular leakage and proliferation. Melatonin is a potent antioxidant capable of protecting variety of retinal cells from oxidative damage. In addition to the pineal gland, the retina produces melatonin. In the current study, we investigated whether melatonin protects against hyperglycemia-induced oxidative injury to Müller cells and explored the potential underlying mechanisms. Our results show that both melatonin membrane receptors, MT1 and MT2, are expressed in cultured primary Müller cells and are upregulated by elevated glucose levels. Both basal and high glucose-induced VEGF production was attenuated by melatonin treatment in a dose-dependent manner. Furthermore, we found that melatonin is a potent activator of Akt in Müller cells. Our findings suggest that in addition to functioning as a direct free radical scavenger, melatonin can elicit cellular signaling pathways that are protective against retinal injury during diabetic retinopathy.

Original languageEnglish (US)
Article numbere50661
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 4 2012
Externally publishedYes

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Cytoprotection
melatonin
Melatonin
diabetic retinopathy
Wounds and Injuries
Diabetic Retinopathy
cells
vascular endothelial growth factors
Vascular Endothelial Growth Factor A
Melatonin MT1 Receptor
Melatonin MT2 Receptor
Glucose
Retinal Vessels
Free Radical Scavengers
Pineal Gland
Cell signaling
pineal body
cell communication
Blindness
glucose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Jiang, T., Chang, Q., Zhao, Z., Yan, S., Wang, L., Cai, J., & Xu, G. (2012). Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells. PLoS One, 7(12), [e50661]. https://doi.org/10.1371/journal.pone.0050661

Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells. / Jiang, Tingting; Chang, Qing; Zhao, Zhenyang; Yan, Saimei; Wang, Ling; Cai, Jiyang; Xu, Gezhi.

In: PLoS One, Vol. 7, No. 12, e50661, 04.12.2012.

Research output: Contribution to journalArticle

Jiang, T, Chang, Q, Zhao, Z, Yan, S, Wang, L, Cai, J & Xu, G 2012, 'Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells', PLoS One, vol. 7, no. 12, e50661. https://doi.org/10.1371/journal.pone.0050661
Jiang, Tingting ; Chang, Qing ; Zhao, Zhenyang ; Yan, Saimei ; Wang, Ling ; Cai, Jiyang ; Xu, Gezhi. / Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells. In: PLoS One. 2012 ; Vol. 7, No. 12.
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