Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide

Miguel Pappolla, Melisa Sos, Rawhi A. Omar, Roger J. Bick, Diane L M Hickson-Bick, Russel J. Reiter, Spiros Efthimiopoulos, Nickolaos K. Robakis

Research output: Contribution to journalArticle

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Abstract

Studies from several laboratories have generated evidence suggesting that oxidative stress is involved in the pathogenesis of Alzheimer's disease (AD). The finding that the amyloid β protein (Aβ) has neurotoxic properties and that such effects are, in part, mediated by free radicals has provided insights into mechanisms of cell death in AD and an avenue to explore new therapeutic approaches. In this study we demonstrate that melatonin, a pineal hormone with recently established antioxidant properties, is remarkably effective in preventing death of cultured neuroblastoma cells as well as oxidative damage and intracellular Ca2+ increases induced by a cytotoxic fragment ofAβ. The effects of melatonin were extremely reproducible and corroborated by multiple quantitative methods, including cell viability studies by confocal laser microscopy, electron microscopy, and measurements of intracellular calcium levels. The importance of this finding is that, in contrast to conventional antioxidants, melatonin has a proposed physiological role in the aging process. Secretion levels of this hormone are decreased in aging and more severely reduced in AD. The reported phenomenon may be of therapeutic relevance in AD.

Original languageEnglish
Pages (from-to)1683-1690
Number of pages8
JournalJournal of Neuroscience
Volume17
Issue number5
StatePublished - Mar 1 1997
Externally publishedYes

Fingerprint

Melatonin
Neuroblastoma
Amyloid
Alzheimer Disease
Cell Death
Peptides
Confocal Microscopy
Antioxidants
Hormones
Serum Amyloid A Protein
Free Radicals
Cultured Cells
Cell Survival
Electron Microscopy
Oxidative Stress
Calcium
Therapeutics

Keywords

  • Aβ toxicity
  • Alzheimer's disease
  • antioxidants
  • melatonin
  • neuronal cells
  • oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pappolla, M., Sos, M., Omar, R. A., Bick, R. J., Hickson-Bick, D. L. M., Reiter, R. J., ... Robakis, N. K. (1997). Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide. Journal of Neuroscience, 17(5), 1683-1690.

Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide. / Pappolla, Miguel; Sos, Melisa; Omar, Rawhi A.; Bick, Roger J.; Hickson-Bick, Diane L M; Reiter, Russel J.; Efthimiopoulos, Spiros; Robakis, Nickolaos K.

In: Journal of Neuroscience, Vol. 17, No. 5, 01.03.1997, p. 1683-1690.

Research output: Contribution to journalArticle

Pappolla, M, Sos, M, Omar, RA, Bick, RJ, Hickson-Bick, DLM, Reiter, RJ, Efthimiopoulos, S & Robakis, NK 1997, 'Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide', Journal of Neuroscience, vol. 17, no. 5, pp. 1683-1690.
Pappolla M, Sos M, Omar RA, Bick RJ, Hickson-Bick DLM, Reiter RJ et al. Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide. Journal of Neuroscience. 1997 Mar 1;17(5):1683-1690.
Pappolla, Miguel ; Sos, Melisa ; Omar, Rawhi A. ; Bick, Roger J. ; Hickson-Bick, Diane L M ; Reiter, Russel J. ; Efthimiopoulos, Spiros ; Robakis, Nickolaos K. / Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 5. pp. 1683-1690.
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