Membrane topology of coronavirus E protein

Junko Maeda, John F. Repass, Akihiko Maeda, Shinji Makino

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Coronavirus small envelope protein E has two known biological functions: it plays a pivotal role in virus envelope formation, and the murine coronavirus E protein induces apoptosis in E protein-expressing cultured cells. The E protein is an integral membrane protein. Its C-terminal region extends cytoplasmically in the infected cell and in the virion toward the interior. The N-terminal two-thirds of the E protein is hydrophobic and lies buried within the membrane, but its orientation in the lipid membrane is not known. Immunofluorescent analyses of cells expressing biologically active murine coronavirus E protein with a hydrophilic short epitope tag at the N-terminus showed that the epitope tag was exposed cytoplasmically. Immunoprecipitation analyses of the purified microsomal membrane vesicles that contain the same tagged E protein revealed the N-terminal epitope tag outside the microsomal membrane vesicles. These analyses demonstrated that the epitope tag at the N-terminus of the E protein was exposed cytoplasmically. Our data were consistent with an E protein topology model, in which the N-terminal two-thirds of the transmembrane domain spans the lipid bilayer twice, exposing the C-terminal region to the cytoplasm or virion interior.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalVirology
Volume281
Issue number2
DOIs
StatePublished - Mar 15 2001

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Coronavirus
Membranes
Proteins
Epitopes
Virion
Lipid Bilayers
Membrane Lipids
Immunoprecipitation
Cultured Cells
Membrane Proteins
Cytoplasm
Apoptosis
Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Membrane topology of coronavirus E protein. / Maeda, Junko; Repass, John F.; Maeda, Akihiko; Makino, Shinji.

In: Virology, Vol. 281, No. 2, 15.03.2001, p. 163-169.

Research output: Contribution to journalArticle

Maeda, J, Repass, JF, Maeda, A & Makino, S 2001, 'Membrane topology of coronavirus E protein', Virology, vol. 281, no. 2, pp. 163-169. https://doi.org/10.1006/viro.2001.0818
Maeda, Junko ; Repass, John F. ; Maeda, Akihiko ; Makino, Shinji. / Membrane topology of coronavirus E protein. In: Virology. 2001 ; Vol. 281, No. 2. pp. 163-169.
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