TY - JOUR
T1 - Mesenchymal stem cell therapy using Pal-KTTKS-enriched carboxylated cellulose improves burn wound in rat model
AU - Rasouli, Mehdi
AU - Shahghasempour, Lida
AU - Shirbaghaee, Zeinab
AU - Hosseinzadeh, Simzar
AU - Abbaszadeh, Hojjat Allah
AU - Fattahi, Roya
AU - Ranjbari, Javad
AU - Soleimani, Masoud
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
PY - 2024/9
Y1 - 2024/9
N2 - Despite the great progress in developing wound dressings, delayed wound closure still remains a global challenge. Thus, developing novel wound dressings and employing advanced strategies, including tissue engineering, are urgently desired. The carboxylated cellulose was developed through the in situ synthesis method and further reinforced by incorporating pal-KTTKS to stimulate collagen synthesis and improve wound healing. The developed composites supported cell adhesion and proliferation and showed good biocompatibility. To boost wound-healing performance, adipose-derived mesenchymal stem cells (MSC) were seeded on the pal-KTTKS-enriched composites to be implanted in a rat model of burn wound healing. Healthy male rats were randomly divided into four groups and wound-healing performance of Vaseline gauze (control), carboxylated cellulose (CBC), pal-KTTKS-enriched CBC (KTTKS-CBC), and MSCs seeded on the KTTKS-CBC composites (MSC-KTTKS-CBC) were evaluated on days 3, 7, and 14 post-implantation. In each group, the designed therapeutic dressings were renewed every 5 days to increase wound-healing performance. We found that KTTKS-CBC and MSC-KTTKS-CBC composites exhibited significantly better wound healing capability, as evidenced by significantly alleviated inflammation, increased collagen deposition, improved angiogenesis, and considerably accelerated wound closure. Nevertheless, the best wound-healing performance was observed in the MSC-KTTKS-CBC groups among all four groups. This research suggests that the MSC-KTTKS-CBC composite offers a great deal of promise as a wound dressing to enhance wound regeneration and expedite wound closure in the clinic. Graphical Abstract: Schematic Illustration Of The Cbc Composites Preparation And Wound Healing Improvement Following Their Implantation. Bacterial Cellulose (bc), Carboxylated Bc (cbc), Kttks-enriched Cbc (kttks-cbc) Mesenchymal Stem Cell- Seeded Kttks-cbc (msc-kttks-cbc).: (Figure presented.)
AB - Despite the great progress in developing wound dressings, delayed wound closure still remains a global challenge. Thus, developing novel wound dressings and employing advanced strategies, including tissue engineering, are urgently desired. The carboxylated cellulose was developed through the in situ synthesis method and further reinforced by incorporating pal-KTTKS to stimulate collagen synthesis and improve wound healing. The developed composites supported cell adhesion and proliferation and showed good biocompatibility. To boost wound-healing performance, adipose-derived mesenchymal stem cells (MSC) were seeded on the pal-KTTKS-enriched composites to be implanted in a rat model of burn wound healing. Healthy male rats were randomly divided into four groups and wound-healing performance of Vaseline gauze (control), carboxylated cellulose (CBC), pal-KTTKS-enriched CBC (KTTKS-CBC), and MSCs seeded on the KTTKS-CBC composites (MSC-KTTKS-CBC) were evaluated on days 3, 7, and 14 post-implantation. In each group, the designed therapeutic dressings were renewed every 5 days to increase wound-healing performance. We found that KTTKS-CBC and MSC-KTTKS-CBC composites exhibited significantly better wound healing capability, as evidenced by significantly alleviated inflammation, increased collagen deposition, improved angiogenesis, and considerably accelerated wound closure. Nevertheless, the best wound-healing performance was observed in the MSC-KTTKS-CBC groups among all four groups. This research suggests that the MSC-KTTKS-CBC composite offers a great deal of promise as a wound dressing to enhance wound regeneration and expedite wound closure in the clinic. Graphical Abstract: Schematic Illustration Of The Cbc Composites Preparation And Wound Healing Improvement Following Their Implantation. Bacterial Cellulose (bc), Carboxylated Bc (cbc), Kttks-enriched Cbc (kttks-cbc) Mesenchymal Stem Cell- Seeded Kttks-cbc (msc-kttks-cbc).: (Figure presented.)
KW - Bacterial cellulose (BC)
KW - Burn
KW - Carboxymethyl cellulose (CMC)
KW - Cell therapy
KW - Mesenchymal stem cell (MSC)
KW - Pal-KTTKS
KW - Regenerative medicine
KW - Scaffold
KW - Tissue engineering
KW - Wound dressing
KW - Wound healing
UR - https://www.scopus.com/pages/publications/85195426988
UR - https://www.scopus.com/pages/publications/85195426988#tab=citedBy
U2 - 10.1007/s00403-024-03082-1
DO - 10.1007/s00403-024-03082-1
M3 - Article
C2 - 38850353
AN - SCOPUS:85195426988
SN - 0340-3696
VL - 316
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 7
M1 - 353
ER -