Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ

W. F. Brechue, K. E. Gropp, Bill Ameredes, D. M. O'Drobinak, W. N. Stainsby, J. W. Harvey

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mechanical and metabolic relationships of muscle lacking phosphofructokinase (PFKD) activity were compared with muscle having normal phosphofructokinase (NORM) activity by using the gastrocnemius-plantaris muscle group with isolated circulation in situ. Muscle contractile properties were similar in both groups. Initial power output (Ẇ) during repetitive tetanic (200 ms, 50 impulses/s) isotonic contractions was similar in both groups; however, Ẇ declined significantly more (30-80%) in PFKD than in NORM muscle over time, with a constant O2 uptake (V̇O2)/Ẇ. Despite similar O2 and substrate delivery, PFKD muscle had a lower V̇O2 (42-55%), less glucose uptake, similar free fatty acid uptake, and lactic acid uptake, rather than output, during contractions. Muscle venous H+ concentration, strong ion difference, and PCO2 increased during contractions, the magnitude of change being smaller in PFKD muscle. Elevating arterial lactate concentration before contractions in PFKD muscle resulted in significant improvements in Ẇ and V̇O2 without altering the acid-base exchange at the muscle. Increasing O2 delivery by increasing arterial O2 concentration in PFKD dogs did not improve Ẇ or V̇O2. We conclude that, despite no inherent mechanical or contractile differences, PFKD muscle has a severely limited oxidative capacity and exaggerated fatigue and blood flow responses to contractions due to limited substrate metabolism resulting from the inability to utilize glycogen and/or glucose.

Original languageEnglish (US)
Pages (from-to)2456-2467
Number of pages12
JournalJournal of Applied Physiology
Volume77
Issue number5
StatePublished - 1994
Externally publishedYes

Fingerprint

Skeletal Muscle
Dogs
Muscles
Phosphofructokinases
Lactic Acid
Isotonic Contraction
Glucose
Glycogen
Nonesterified Fatty Acids
Fatigue
Ions
Acids

Keywords

  • acid-base balance
  • carbohydrate metabolism
  • control of blood flow
  • fatigue
  • lactic acid
  • oxygen uptake
  • power output
  • strong ion difference

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Brechue, W. F., Gropp, K. E., Ameredes, B., O'Drobinak, D. M., Stainsby, W. N., & Harvey, J. W. (1994). Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ. Journal of Applied Physiology, 77(5), 2456-2467.

Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ. / Brechue, W. F.; Gropp, K. E.; Ameredes, Bill; O'Drobinak, D. M.; Stainsby, W. N.; Harvey, J. W.

In: Journal of Applied Physiology, Vol. 77, No. 5, 1994, p. 2456-2467.

Research output: Contribution to journalArticle

Brechue, WF, Gropp, KE, Ameredes, B, O'Drobinak, DM, Stainsby, WN & Harvey, JW 1994, 'Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ', Journal of Applied Physiology, vol. 77, no. 5, pp. 2456-2467.
Brechue WF, Gropp KE, Ameredes B, O'Drobinak DM, Stainsby WN, Harvey JW. Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ. Journal of Applied Physiology. 1994;77(5):2456-2467.
Brechue, W. F. ; Gropp, K. E. ; Ameredes, Bill ; O'Drobinak, D. M. ; Stainsby, W. N. ; Harvey, J. W. / Metabolic and work capacity of skeletal muscle of PFK-deficient dogs studied in situ. In: Journal of Applied Physiology. 1994 ; Vol. 77, No. 5. pp. 2456-2467.
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