Metabolism of L-arginine and the no pathway in septic children

Z. Argaman, N. Noviski, L. Castillo-Rosas, R. Ritz, V. Rigatti, A. Ajami, V. R. Young, Leticia Castillo

Research output: Contribution to journalArticlepeer-review

Abstract

To determine the in vivo status and regulatory mechanisms of arginine and related metabolic pathways in septic children, we conducted an 8h primed constant intravenous infusion of L-[13C] leu and L-[guanidino 15N2, 5,5,2H2] arg in 10 septic children (6 to 16y; 20-60kg). Four patients also received a second infusion of L-[13C] arg and L-[13C ureido 5,5,2H2] cit the next day. Frequent blood, breath, urine samples and indirect calorimetry were obtained. Isotopic enrichments were measured by GC-MS and IR-MS. Six healthy young adults (18-21y; 60-80kg); receiving adequate protein and energy intake for a week served as controls. Group De novo Arg Synthesis Arg Ox NO Synthesis Arg to Cit Plasma Arg to NO3-Septic 9.6±31 16.9±3*1.6±0.9*11.7±5*Healthy 9.2±1.4 7.6±0.4 0.96±0.1 1.7±4 1 All Values are Mean ± Sem; μmol.kg.h;*p<0.05 Homeostasis of arg metabolism in septic patients is impaired. Arg oxidation is greater than synthesis, resulting in a negative arg balance. NO synthesis was increased and appeared to reflect severity of disease. Plasma arg for NO synthesis is utilized to a greater extent in septic patients.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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