Metabolism of prostaglandin A1 by hepatic microsomal monooxygenase P-450 system in the guinea pig and rat

David Kupfer; Javier Navarro

doi:10.1016/0024-3205(76)90328-3

Metabolism of prostaglandin A₁ by hepatic microsomal monooxygenase P-450 system in the guinea pig and rat

David Kupfer, Javier Navarro

Research output: Contribution to journal › Article

13 Scopus citations

Abstract

This study demonstrates the metabolic transformation of prostaglandin A₁ (PGA₁) by guinea pig and rat liver microsomes. The transformation, which required NADPH and oxygen, yielded polar (presumably hydroxylated) products. Incubations with guinea pig liver microsomes yielded one zone of product on tlc, whereas rat liver microsomes produced two discernable metabolic zones. It was demonstrated that PGA₁ metabolism in the guinea pig and the rat was inhibited by the addition of SKF-525A, metyrapone, carbon monoxide and cytochrome C; nicotinamide (10 mM) inhibited only the guinea pig system. These findings indicate that the enzymatic activity responsible for PGA₁ metabolism is composed of a typical cytochrome P-450 monooxygenase system.

Original language	English (US)
Pages (from-to)	507-513
Number of pages	7
Journal	Life Sciences
Volume	18
Issue number	5
DOIs	https://doi.org/10.1016/0024-3205(76)90328-3
State	Published - Mar 1 1976
Externally published	Yes

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ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Kupfer, D., & Navarro, J. (1976). Metabolism of prostaglandin A₁ by hepatic microsomal monooxygenase P-450 system in the guinea pig and rat. Life Sciences, 18(5), 507-513. https://doi.org/10.1016/0024-3205(76)90328-3

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AB - This study demonstrates the metabolic transformation of prostaglandin A1 (PGA1) by guinea pig and rat liver microsomes. The transformation, which required NADPH and oxygen, yielded polar (presumably hydroxylated) products. Incubations with guinea pig liver microsomes yielded one zone of product on tlc, whereas rat liver microsomes produced two discernable metabolic zones. It was demonstrated that PGA1 metabolism in the guinea pig and the rat was inhibited by the addition of SKF-525A, metyrapone, carbon monoxide and cytochrome C; nicotinamide (10 mM) inhibited only the guinea pig system. These findings indicate that the enzymatic activity responsible for PGA1 metabolism is composed of a typical cytochrome P-450 monooxygenase system.

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10.1016/0024-3205(76)90328-3