Metagenomic Analysis of the Airborne Environment in Urban Spaces

Nicholas A. Be, James B. Thissen, Viacheslav Y. Fofanov, Jonathan E. Allen, Mark Rojas, George Golovko, Yuriy Fofanov, Heather Koshinsky, Crystal J. Jaing

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The organisms in aerosol microenvironments, especially densely populated urban areas, are relevant to maintenance of public health and detection of potential epidemic or biothreat agents. To examine aerosolized microorganisms in this environment, we performed sequencing on the material from an urban aerosol surveillance program. Whole metagenome sequencing was applied to DNA extracted from air filters obtained during periods from each of the four seasons. The composition of bacteria, plants, fungi, invertebrates, and viruses demonstrated distinct temporal shifts. Bacillus thuringiensis serovar kurstaki was detected in samples known to be exposed to aerosolized spores, illustrating the potential utility of this approach for identification of intentionally introduced microbial agents. Together, these data demonstrate the temporally dependent metagenomic complexity of urban aerosols and the potential of genomic analytical techniques for biosurveillance and monitoring of threats to public health.

Original languageEnglish (US)
Pages (from-to)346-355
Number of pages10
JournalMicrobial Ecology
Volume69
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Metagenomics
aerosols
Aerosols
aerosol
Biosurveillance
public health
Public Health
air filters
Air Filters
Metagenome
Bacillus thuringiensis subsp. kurstaki
Bacillus thuringiensis
monitoring
Invertebrates
Spores
urban areas
analytical methods
spore
analytical method
genomics

Keywords

  • Aerosol microbiology
  • Airborne bacteria
  • Metagenomics
  • Microbiome
  • Urban air

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science
  • Medicine(all)

Cite this

Be, N. A., Thissen, J. B., Fofanov, V. Y., Allen, J. E., Rojas, M., Golovko, G., ... Jaing, C. J. (2014). Metagenomic Analysis of the Airborne Environment in Urban Spaces. Microbial Ecology, 69(2), 346-355. https://doi.org/10.1007/s00248-014-0517-z

Metagenomic Analysis of the Airborne Environment in Urban Spaces. / Be, Nicholas A.; Thissen, James B.; Fofanov, Viacheslav Y.; Allen, Jonathan E.; Rojas, Mark; Golovko, George; Fofanov, Yuriy; Koshinsky, Heather; Jaing, Crystal J.

In: Microbial Ecology, Vol. 69, No. 2, 2014, p. 346-355.

Research output: Contribution to journalArticle

Be, NA, Thissen, JB, Fofanov, VY, Allen, JE, Rojas, M, Golovko, G, Fofanov, Y, Koshinsky, H & Jaing, CJ 2014, 'Metagenomic Analysis of the Airborne Environment in Urban Spaces', Microbial Ecology, vol. 69, no. 2, pp. 346-355. https://doi.org/10.1007/s00248-014-0517-z
Be, Nicholas A. ; Thissen, James B. ; Fofanov, Viacheslav Y. ; Allen, Jonathan E. ; Rojas, Mark ; Golovko, George ; Fofanov, Yuriy ; Koshinsky, Heather ; Jaing, Crystal J. / Metagenomic Analysis of the Airborne Environment in Urban Spaces. In: Microbial Ecology. 2014 ; Vol. 69, No. 2. pp. 346-355.
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