Metagenomic identification and sequence analysis of a Teschovirus A-related virus in porcine feces in Japan, 2014–2016

Mami Oba, Yuki Naoi, Mika Ito, Tsuneyuki Masuda, Yukie Katayama, Shoichi Sakaguchi, Tsutomu Omatsu, Tetsuya Furuya, Hiroshi Yamasato, Fujiko Sunaga, Shinji Makino, Tetsuya Mizutani, Makoto Nagai

Research output: Contribution to journalArticle

Abstract

Porcine Teschoviruses (PTVs) are associated with polioencephalomyelitis and various diseases, including reproductive and gastrointestinal disorders, of pigs and wild boars, and are also detected in the feces of healthy pigs. The genus Teschovirus contains a single species Teschovirus A that currently includes 13 serotypes. In the present study, we identified novel PTVs that are distantly related to Teschovirus A and were found in fecal samples of pigs with or without diarrhea in Japan. Phylogenetic analysis of amino acid (aa) sequences of the complete coding region revealed that these newly identified viruses did not cluster with any strains of PTVs or other strains within the picornavirus supergroup 1, suggesting that the viruses may not belong to Teschovirus A or any genus of the family Picornaviridae. These novel PTVs share a type IV internal ribosomal entry site and conserved characteristic motifs in the coding region, yet exhibit 62.2–79.0%, 86.6–92.8%, 77.1–81.0%, and 84.3–86.7% aa identities to PTV strains in P1, 2C, 3C, and 3D regions, respectively. In contrast, PTV 1–13 strains of the Teschovirus A share 76.5–92.1%, 88.1–99.7%, 93.2–100%, and 95.8–100% aa identities in the P1, 2C, 3C, and 3D, respectively, within the species. These data imply that the newly identified viruses belong to teschoviruses, and may represent a novel species in the genus Teschovirus.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalInfection, Genetics and Evolution
Volume66
DOIs
StatePublished - Dec 1 2018

Fingerprint

Teschovirus
Porcine teschovirus
Metagenomics
Feces
pig
feces
Sequence Analysis
virus
Japan
Swine
sequence analysis
amino acid
Viruses
viruses
swine
Picornaviridae
phylogenetics
reproductive disorders
amino acids
wild boars

Keywords

  • Complete genome analysis
  • Metagenomics approach
  • Novel Teschovirus
  • Porcine feces

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Metagenomic identification and sequence analysis of a Teschovirus A-related virus in porcine feces in Japan, 2014–2016. / Oba, Mami; Naoi, Yuki; Ito, Mika; Masuda, Tsuneyuki; Katayama, Yukie; Sakaguchi, Shoichi; Omatsu, Tsutomu; Furuya, Tetsuya; Yamasato, Hiroshi; Sunaga, Fujiko; Makino, Shinji; Mizutani, Tetsuya; Nagai, Makoto.

In: Infection, Genetics and Evolution, Vol. 66, 01.12.2018, p. 210-216.

Research output: Contribution to journalArticle

Oba, M, Naoi, Y, Ito, M, Masuda, T, Katayama, Y, Sakaguchi, S, Omatsu, T, Furuya, T, Yamasato, H, Sunaga, F, Makino, S, Mizutani, T & Nagai, M 2018, 'Metagenomic identification and sequence analysis of a Teschovirus A-related virus in porcine feces in Japan, 2014–2016', Infection, Genetics and Evolution, vol. 66, pp. 210-216. https://doi.org/10.1016/j.meegid.2018.10.004
Oba, Mami ; Naoi, Yuki ; Ito, Mika ; Masuda, Tsuneyuki ; Katayama, Yukie ; Sakaguchi, Shoichi ; Omatsu, Tsutomu ; Furuya, Tetsuya ; Yamasato, Hiroshi ; Sunaga, Fujiko ; Makino, Shinji ; Mizutani, Tetsuya ; Nagai, Makoto. / Metagenomic identification and sequence analysis of a Teschovirus A-related virus in porcine feces in Japan, 2014–2016. In: Infection, Genetics and Evolution. 2018 ; Vol. 66. pp. 210-216.
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abstract = "Porcine Teschoviruses (PTVs) are associated with polioencephalomyelitis and various diseases, including reproductive and gastrointestinal disorders, of pigs and wild boars, and are also detected in the feces of healthy pigs. The genus Teschovirus contains a single species Teschovirus A that currently includes 13 serotypes. In the present study, we identified novel PTVs that are distantly related to Teschovirus A and were found in fecal samples of pigs with or without diarrhea in Japan. Phylogenetic analysis of amino acid (aa) sequences of the complete coding region revealed that these newly identified viruses did not cluster with any strains of PTVs or other strains within the picornavirus supergroup 1, suggesting that the viruses may not belong to Teschovirus A or any genus of the family Picornaviridae. These novel PTVs share a type IV internal ribosomal entry site and conserved characteristic motifs in the coding region, yet exhibit 62.2–79.0{\%}, 86.6–92.8{\%}, 77.1–81.0{\%}, and 84.3–86.7{\%} aa identities to PTV strains in P1, 2C, 3C, and 3D regions, respectively. In contrast, PTV 1–13 strains of the Teschovirus A share 76.5–92.1{\%}, 88.1–99.7{\%}, 93.2–100{\%}, and 95.8–100{\%} aa identities in the P1, 2C, 3C, and 3D, respectively, within the species. These data imply that the newly identified viruses belong to teschoviruses, and may represent a novel species in the genus Teschovirus.",
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AU - Oba, Mami

AU - Naoi, Yuki

AU - Ito, Mika

AU - Masuda, Tsuneyuki

AU - Katayama, Yukie

AU - Sakaguchi, Shoichi

AU - Omatsu, Tsutomu

AU - Furuya, Tetsuya

AU - Yamasato, Hiroshi

AU - Sunaga, Fujiko

AU - Makino, Shinji

AU - Mizutani, Tetsuya

AU - Nagai, Makoto

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N2 - Porcine Teschoviruses (PTVs) are associated with polioencephalomyelitis and various diseases, including reproductive and gastrointestinal disorders, of pigs and wild boars, and are also detected in the feces of healthy pigs. The genus Teschovirus contains a single species Teschovirus A that currently includes 13 serotypes. In the present study, we identified novel PTVs that are distantly related to Teschovirus A and were found in fecal samples of pigs with or without diarrhea in Japan. Phylogenetic analysis of amino acid (aa) sequences of the complete coding region revealed that these newly identified viruses did not cluster with any strains of PTVs or other strains within the picornavirus supergroup 1, suggesting that the viruses may not belong to Teschovirus A or any genus of the family Picornaviridae. These novel PTVs share a type IV internal ribosomal entry site and conserved characteristic motifs in the coding region, yet exhibit 62.2–79.0%, 86.6–92.8%, 77.1–81.0%, and 84.3–86.7% aa identities to PTV strains in P1, 2C, 3C, and 3D regions, respectively. In contrast, PTV 1–13 strains of the Teschovirus A share 76.5–92.1%, 88.1–99.7%, 93.2–100%, and 95.8–100% aa identities in the P1, 2C, 3C, and 3D, respectively, within the species. These data imply that the newly identified viruses belong to teschoviruses, and may represent a novel species in the genus Teschovirus.

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