Metagenomic profile of the viral communities in rhipicephalus spp. ticks from Yunnan, China

Han Xia, Cheng Hu, Dake Zhang, Shuang Tang, Zhong Zhang, Zheng Kou, Zhaojun Fan, Dennis Bente, Changqing Zeng, Tianxian Li

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Besides mosquitoes, ticks are regarded as the primary source of vector-borne infectious diseases. Indeed, a wide variety of severe infectious human diseases, including those involving viruses, are transmitted by ticks in many parts of the world. To date, there are no published reports on the use of next-generation sequencing for studying viral diversity in ticks or discovering new viruses in these arthropods from China. Here, Ion-torrent sequencing was used to investigate the presence of viruses in three Rhipicephalus spp. tick pools (NY-11, NY-13, and MM-13) collected from the Menglian district of Yunnan, China. The sequencing run resulted in 3,641,088, 3,106,733, and 3,871,851 reads in each tick pool after trimming. Reads and assembled contiguous sequences (contigs) were subject to basic local alignment search tool analysis against the GenBank database. Large numbers of reads and contigs related to known viral sequences corresponding to a broad range of viral families were identified. Some of the sequences originated from viruses that have not been described previously in ticks. Our findings will facilitate better understanding of the tick virome, and add to our current knowledge of disease-causing viruses in ticks living under natural conditions.

Original languageEnglish (US)
Article numbere0121609
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 23 2015

Fingerprint

Rhipicephalus
Metagenomics
Ticks
Viruses
ticks
China
viruses
Trimming
Disease Vectors
Arthropods
Ions
Nucleic Acid Databases
Virus Diseases
Culicidae
human diseases
infectious diseases
Communicable Diseases
arthropods
Databases
ions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Xia, H., Hu, C., Zhang, D., Tang, S., Zhang, Z., Kou, Z., ... Li, T. (2015). Metagenomic profile of the viral communities in rhipicephalus spp. ticks from Yunnan, China. PLoS One, 10(3), [e0121609]. https://doi.org/10.1371/journal.pone.0121609

Metagenomic profile of the viral communities in rhipicephalus spp. ticks from Yunnan, China. / Xia, Han; Hu, Cheng; Zhang, Dake; Tang, Shuang; Zhang, Zhong; Kou, Zheng; Fan, Zhaojun; Bente, Dennis; Zeng, Changqing; Li, Tianxian.

In: PLoS One, Vol. 10, No. 3, e0121609, 23.03.2015.

Research output: Contribution to journalArticle

Xia, H, Hu, C, Zhang, D, Tang, S, Zhang, Z, Kou, Z, Fan, Z, Bente, D, Zeng, C & Li, T 2015, 'Metagenomic profile of the viral communities in rhipicephalus spp. ticks from Yunnan, China', PLoS One, vol. 10, no. 3, e0121609. https://doi.org/10.1371/journal.pone.0121609
Xia, Han ; Hu, Cheng ; Zhang, Dake ; Tang, Shuang ; Zhang, Zhong ; Kou, Zheng ; Fan, Zhaojun ; Bente, Dennis ; Zeng, Changqing ; Li, Tianxian. / Metagenomic profile of the viral communities in rhipicephalus spp. ticks from Yunnan, China. In: PLoS One. 2015 ; Vol. 10, No. 3.
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