Metastatic tumor antigen 1 short form (MTA1s) associates with casein kinase I-γ2, an estrogen-responsive kinase

Recent studies have shown that metastasis-associated protein-1 short form (MTA1s) - metastatic tumor antigen 1 short form sequesters estrogen receptor-alpha (ER-α) in the cytoplasm of breast cancer cells. Using a yeast two-hybrid screening to clone MTA1s-interacting proteins, we identified casein kinase I-gamma 2 (CKI-γ2, a ubiquitously expressed cytoplasmic kinase) as an MTA1s-binding protein. We show that MTA1s interacts with CKI-γ2 both in vitro and in vivo and colocalizes in the cytoplasm. In addition, we found that CKI-γ2 can phospliorylate MTA1s, but not ER, in an antiestrogen-dependent manner and that estrogen stimulates CKI-γ2 activity that could be effectively blocked by a specific inhibitor of CKI. CKI-γ2 could further potentiate the ER corepressive function of MTA1s. Kinase dead CK1-γ2 could not repress estrogen-induced ER transactivation functions. Results from mutagenesis studies suggest that substitution of the serine residue at 321 to alanine, which is a possible CKI-γ2 phopshorylation site in MTA1s, results in a significant reduction in the ability of MTA1s to repress ER transactivation. These findings identified MTA1s as a target of CKI-γ2, and provided new evidence to suggest that CKI-γ2 phosphorylates and modulates the functions of MTA1s, and that these extranuclear effects of estrogen might have important implications in regulating the functions of MTA1s in human mammary epithelial and cancer cells.