In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60°C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.
|Original language||English (US)|
|Number of pages||2|
|Journal||Journal of Clinical Microbiology|
|State||Published - Jan 1 1989|
ASJC Scopus subject areas
- Microbiology (medical)