Method for improved extraction of DNA from Nocardia asteroides

M. J. Loeffelholz, D. R. Scholl

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60°C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.

Original languageEnglish (US)
Pages (from-to)1880-1881
Number of pages2
JournalJournal of Clinical Microbiology
Volume27
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Nocardia asteroides
Sodium Dodecyl Sulfate
DNA
DNA Probes
Centrifugation
Nucleic Acids
Suspensions

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Loeffelholz, M. J., & Scholl, D. R. (1989). Method for improved extraction of DNA from Nocardia asteroides. Journal of Clinical Microbiology, 27(8), 1880-1881.

Method for improved extraction of DNA from Nocardia asteroides. / Loeffelholz, M. J.; Scholl, D. R.

In: Journal of Clinical Microbiology, Vol. 27, No. 8, 1989, p. 1880-1881.

Research output: Contribution to journalArticle

Loeffelholz, MJ & Scholl, DR 1989, 'Method for improved extraction of DNA from Nocardia asteroides', Journal of Clinical Microbiology, vol. 27, no. 8, pp. 1880-1881.
Loeffelholz, M. J. ; Scholl, D. R. / Method for improved extraction of DNA from Nocardia asteroides. In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 8. pp. 1880-1881.
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