Method for improved extraction of DNA from Nocardia asteroides

M. J. Loeffelholz, D. R. Scholl

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60°C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.

Original languageEnglish (US)
Pages (from-to)1880-1881
Number of pages2
JournalJournal of Clinical Microbiology
Volume27
Issue number8
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology (medical)

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