Abstract
In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60°C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1880-1881 |
| Number of pages | 2 |
| Journal | Journal of Clinical Microbiology |
| Volume | 27 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1989 |
| Externally published | Yes |
ASJC Scopus subject areas
- Microbiology (medical)