Method for improved extraction of DNA from Nocardia asteroides

M. J. Loeffelholz, D. R. Scholl

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Abstract

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60°C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.

Original languageEnglish (US)
Pages (from-to)1880-1881
Number of pages2
JournalJournal of Clinical Microbiology
Volume27
Issue number8
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Loeffelholz, M. J., & Scholl, D. R. (1989). Method for improved extraction of DNA from Nocardia asteroides. Journal of Clinical Microbiology, 27(8), 1880-1881.