Method for lipidomic analysis

p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells

Huan He, Charles A. Conrad, Carol L. Nilsson, Yongjie Ji, Tanner M. Schaub, Alan G. Marshall, Mark Emmett

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Lipidomics can complement genomics and proteomics by providing new insight into dynamic changes in biomembranes; however, few reports in the literature have explored, on an organism-wide scale, the functional link between nonenzymatic proteins and cellular lipids. Here, we report changes induced by adenovirus-delivered wildtype p53 gene and chemotherapy of U87 MG glioblastoma cells, a treatment known to trigger apoptosis and cell cycle arrest. We compare polar lipid changes in treated cells and control cells by use of a novel, sensitive method that employs lipid extraction, one-step liquid chromatography separation, high-resolution mass analysis, and Kendrick mass defect analysis. Nano-LC FT-ICR MS and quadrupole linear ion trap MS/MS analysis of polar lipids yields hundreds of unique assignments of glyco- and phospholipids at sub-ppm mass accuracy and high resolving power (m/Δm50% = 200 000 at m/z 400) at 1 s/scan. MS/MS data confirm molecular structures in many instances. Sulfatides are most highly modulated by wild-type p53 treatment The treatment also leads to an increase in phospholipids such as phosphatidyl inositols, phosphatidyl serines, phosphatidyl glycerols, and phosphatidyl ethanolamines. An increase in hydroxylated phospholipids is especially noteworthy. Also, a decrease in the longer chain gangliosides, GD1 and GM1b, is observed in wildtype p53 (treated) cells.

Original languageEnglish (US)
Pages (from-to)8423-8430
Number of pages8
JournalAnalytical Chemistry
Volume79
Issue number22
DOIs
StatePublished - Nov 15 2007
Externally publishedYes

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Sulfoglycosphingolipids
Gangliosides
Phospholipids
Modulation
Lipids
Chemical analysis
Ethanolamines
Cells
Phosphatidylglycerols
Chemotherapy
Cefotaxime
Phosphatidylserines
Glycolipids
Liquid chromatography
Optical resolving power
Phosphatidylinositols
Molecular structure
Genes
Ions
Apoptosis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Method for lipidomic analysis : p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells. / He, Huan; Conrad, Charles A.; Nilsson, Carol L.; Ji, Yongjie; Schaub, Tanner M.; Marshall, Alan G.; Emmett, Mark.

In: Analytical Chemistry, Vol. 79, No. 22, 15.11.2007, p. 8423-8430.

Research output: Contribution to journalArticle

He, Huan ; Conrad, Charles A. ; Nilsson, Carol L. ; Ji, Yongjie ; Schaub, Tanner M. ; Marshall, Alan G. ; Emmett, Mark. / Method for lipidomic analysis : p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells. In: Analytical Chemistry. 2007 ; Vol. 79, No. 22. pp. 8423-8430.
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