Methods for the study of alternative splicing

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Alternative splicing multiplies the coding capacity of genes, which provides unparalleled complexity to the transcriptome and proteome. This increased complexity has clear repercussions for the regulation of gene expression in many organisms and for the balance between human health and disease. This significance, which has only recently been fully understood, has led to a growing number of studies that address the mechanisms that control alternative splicing decisions and the biological consequences of alternative RNA and protein isoforms. These studies have adapted or developed new methods and protocols that span computational biology, the biochemical analysis of in vitro reactions, model tissue culture systems, and in vivo studies. Many of these methods have been assembled in this volume to assist a newcomer to the study of alternative splicing and to expand the methodologies of laboratories already in the field.

Original languageEnglish (US)
Pages (from-to)289-291
Number of pages3
JournalMethods
Volume37
Issue number4
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Alternative Splicing
RNA Isoforms
Tissue culture
Gene Expression Regulation
Proteome
Computational Biology
Transcriptome
Gene expression
Protein Isoforms
Genes
Health

Keywords

  • Alternative splicing
  • Pre-mRNAs
  • Splicing

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Methods for the study of alternative splicing. / Garcia-Blanco, Mariano.

In: Methods, Vol. 37, No. 4, 12.2005, p. 289-291.

Research output: Contribution to journalArticle

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