Methods to study monocyte migration induced by HIV-infected cells

Vasudev R. Rao, Eliseo Eugenin, Joan W. Berman, Vinayaka R. Prasad

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

Original languageEnglish (US)
Title of host publicationHIV Protocols
EditorsVinayaka R. Prasad, Ganjam V. Kalpana
Pages295-309
Number of pages15
DOIs
StatePublished - Feb 23 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume485
ISSN (Print)1064-3745

Fingerprint

Monocytes
Chemokines
HIV
Human Immunodeficiency Virus tat Gene Products
AIDS Dementia Complex
HIV-1
tat Gene Products
Brain
Phagocytes
Recombinant Proteins
HIV Infections

Keywords

  • HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Rao, V. R., Eugenin, E., Berman, J. W., & Prasad, V. R. (2009). Methods to study monocyte migration induced by HIV-infected cells. In V. R. Prasad, & G. V. Kalpana (Eds.), HIV Protocols (pp. 295-309). (Methods in Molecular Biology; Vol. 485). https://doi.org/10.1007/978-1-59745-170-3_20

Methods to study monocyte migration induced by HIV-infected cells. / Rao, Vasudev R.; Eugenin, Eliseo; Berman, Joan W.; Prasad, Vinayaka R.

HIV Protocols. ed. / Vinayaka R. Prasad; Ganjam V. Kalpana. 2009. p. 295-309 (Methods in Molecular Biology; Vol. 485).

Research output: Chapter in Book/Report/Conference proceedingChapter

Rao, VR, Eugenin, E, Berman, JW & Prasad, VR 2009, Methods to study monocyte migration induced by HIV-infected cells. in VR Prasad & GV Kalpana (eds), HIV Protocols. Methods in Molecular Biology, vol. 485, pp. 295-309. https://doi.org/10.1007/978-1-59745-170-3_20
Rao VR, Eugenin E, Berman JW, Prasad VR. Methods to study monocyte migration induced by HIV-infected cells. In Prasad VR, Kalpana GV, editors, HIV Protocols. 2009. p. 295-309. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-170-3_20
Rao, Vasudev R. ; Eugenin, Eliseo ; Berman, Joan W. ; Prasad, Vinayaka R. / Methods to study monocyte migration induced by HIV-infected cells. HIV Protocols. editor / Vinayaka R. Prasad ; Ganjam V. Kalpana. 2009. pp. 295-309 (Methods in Molecular Biology).
@inbook{7905540901eb4832bd1a59ac6508832d,
title = "Methods to study monocyte migration induced by HIV-infected cells",
abstract = "HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.",
keywords = "HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines",
author = "Rao, {Vasudev R.} and Eliseo Eugenin and Berman, {Joan W.} and Prasad, {Vinayaka R.}",
year = "2009",
month = "2",
day = "23",
doi = "10.1007/978-1-59745-170-3_20",
language = "English (US)",
isbn = "9781588298591",
series = "Methods in Molecular Biology",
pages = "295--309",
editor = "Prasad, {Vinayaka R.} and Kalpana, {Ganjam V.}",
booktitle = "HIV Protocols",

}

TY - CHAP

T1 - Methods to study monocyte migration induced by HIV-infected cells

AU - Rao, Vasudev R.

AU - Eugenin, Eliseo

AU - Berman, Joan W.

AU - Prasad, Vinayaka R.

PY - 2009/2/23

Y1 - 2009/2/23

N2 - HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

AB - HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

KW - HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines

UR - http://www.scopus.com/inward/record.url?scp=60349113430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60349113430&partnerID=8YFLogxK

U2 - 10.1007/978-1-59745-170-3_20

DO - 10.1007/978-1-59745-170-3_20

M3 - Chapter

C2 - 19020833

AN - SCOPUS:60349113430

SN - 9781588298591

T3 - Methods in Molecular Biology

SP - 295

EP - 309

BT - HIV Protocols

A2 - Prasad, Vinayaka R.

A2 - Kalpana, Ganjam V.

ER -