Methods to study monocyte migration induced by HIV-infected cells

Vasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman, Vinayaka R. Prasad

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

Original languageEnglish (US)
Title of host publicationHIV Protocols
EditorsVinayaka R. Prasad, Ganjam V. Kalpana
Number of pages15
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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