Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller, Julia Sable, Philip Goelet, Michael P. Sheetz, Virginia W. Cornish

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

Original languageEnglish (US)
Pages (from-to)1672-1675
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number13
StatePublished - Mar 19 2004
Externally publishedYes


  • Bioorganic chemistry
  • Dihydrofolate reductase
  • Fluorescent probes
  • Methotrexate
  • Proteins

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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