Methotrexate conjugates

A molecular in vivo protein tag

Lawrence W. Miller, Julia Sable, Philip Goelet, Michael Sheetz, Virginia W. Cornish

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

Original languageEnglish (US)
Pages (from-to)1672-1675
Number of pages4
JournalAngewandte Chemie - International Edition
Volume43
Issue number13
DOIs
StatePublished - Mar 19 2004
Externally publishedYes

Fingerprint

Methotrexate
Labeling
Cytology
Proteins
Tetrahydrofolate Dehydrogenase
Ligands
Cells
Molecules
Oxidoreductases

Keywords

  • Bioorganic chemistry
  • Dihydrofolate reductase
  • Fluorescent probes
  • Methotrexate
  • Proteins

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Methotrexate conjugates : A molecular in vivo protein tag. / Miller, Lawrence W.; Sable, Julia; Goelet, Philip; Sheetz, Michael; Cornish, Virginia W.

In: Angewandte Chemie - International Edition, Vol. 43, No. 13, 19.03.2004, p. 1672-1675.

Research output: Contribution to journalArticle

Miller, Lawrence W. ; Sable, Julia ; Goelet, Philip ; Sheetz, Michael ; Cornish, Virginia W. / Methotrexate conjugates : A molecular in vivo protein tag. In: Angewandte Chemie - International Edition. 2004 ; Vol. 43, No. 13. pp. 1672-1675.
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