Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller, Julia Sable, Philip Goelet, Michael Sheetz, Virginia W. Cornish

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

Original languageEnglish (US)
Pages (from-to)1672-1675
Number of pages4
JournalAngewandte Chemie - International Edition
Volume43
Issue number13
DOIs
StatePublished - Mar 19 2004
Externally publishedYes

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Keywords

  • Bioorganic chemistry
  • Dihydrofolate reductase
  • Fluorescent probes
  • Methotrexate
  • Proteins

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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