Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller; Julia Sable; Philip Goelet; Michael P. Sheetz; Virginia W. Cornish

doi:10.1002/anie.200352852

Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller
, Julia Sable
, Philip Goelet
, Michael P. Sheetz
, Virginia W. Cornish

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

Original language	English (US)
Pages (from-to)	1672-1675
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	13
DOIs	https://doi.org/10.1002/anie.200352852
State	Published - Mar 19 2004
Externally published	Yes

Keywords

Bioorganic chemistry
Dihydrofolate reductase
Fluorescent probes
Methotrexate
Proteins

ASJC Scopus subject areas

Catalysis
General Chemistry

Access to Document

10.1002/anie.200352852

Cite this

@article{fbcbfcf8838d426da09c33663e57fe8d,

title = "Methotrexate conjugates: A molecular in vivo protein tag",

abstract = "The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).",

keywords = "Bioorganic chemistry, Dihydrofolate reductase, Fluorescent probes, Methotrexate, Proteins",

author = "Miller, \{Lawrence W.\} and Julia Sable and Philip Goelet and Sheetz, \{Michael P.\} and Cornish, \{Virginia W.\}",

year = "2004",

month = mar,

day = "19",

doi = "10.1002/anie.200352852",

language = "English (US)",

volume = "43",

pages = "1672--1675",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "13",

}

TY - JOUR

T1 - Methotrexate conjugates

T2 - A molecular in vivo protein tag

AU - Miller, Lawrence W.

AU - Sable, Julia

AU - Goelet, Philip

AU - Sheetz, Michael P.

AU - Cornish, Virginia W.

PY - 2004/3/19

Y1 - 2004/3/19

N2 - The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

AB - The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

KW - Bioorganic chemistry

KW - Dihydrofolate reductase

KW - Fluorescent probes

KW - Methotrexate

KW - Proteins

UR - https://www.scopus.com/pages/publications/3042584357

UR - https://www.scopus.com/pages/publications/3042584357#tab=citedBy

U2 - 10.1002/anie.200352852

DO - 10.1002/anie.200352852

M3 - Article

C2 - 15038033

AN - SCOPUS:3042584357

SN - 1433-7851

VL - 43

SP - 1672

EP - 1675

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 13

ER -

Methotrexate conjugates: A molecular in vivo protein tag

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this