Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems

Jang Gi Choi, Su Hyun Mun, Harendra S. Chahar, Preeti Bharaj, Ok Hwa Kang, Se Gun Kim, Dong Won Shin, Dong Yeul Kwon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Galla rhois is a commonly used traditional medicine for the treatment of pathogenic bacteria in Korea as well as in other parts of Asia. Methyl gallate (MG), a major component of Galla Rhois, exhibits strong antibacterial activity, but its mechanism of action against Salmonella spp. is unclear. In the present study, we investigated the antibacterial actions of MG against Salmonella. The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of MG against Salmonella strains ranged from 3.9 to 125 μg/ml. In vitro bacterial viability test indicated that MG significantly decreased the viability of Salmonella over 40% when combined with ATPase inhibitors. The time-kill curves showed that a combined MG and ATPase inhibitors (DCCD and NaN3) treatment reduced the bacterial counts dramatically after 24 h. Oral administration of MG showed a strong anti-bacterial activity against WS-5 infected BALB/c mice. In contrast to the untreated Salmonella infected control animals, MG treated groups showed no clinical symptoms of the disease, such as lethargy and liver damage. It was observed that MG treatment significantly increased the survival of animals from Salmonella infection, while in untreated groups all animal succumbed to disease by the sixth day post infection. Thus, the present study demonstrates the therapeutic ability of MG against Salmonella infections.

Original languageEnglish (US)
Article numbere102697
JournalPLoS One
Volume9
Issue number7
DOIs
StatePublished - Jul 21 2014
Externally publishedYes

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Salmonella
Salmonella Infections
salmonellosis
adenosinetriphosphatase
viability
Animals
animals
Animal Salmonella Infections
traditional medicine
Adenosine Triphosphatases
microbial activity
plate count
oral administration
Exanthema Subitum
signs and symptoms (animals and humans)
Korean Peninsula
mechanism of action
Dicyclohexylcarbodiimide
Microbial Viability
methyl gallate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. / Choi, Jang Gi; Mun, Su Hyun; Chahar, Harendra S.; Bharaj, Preeti; Kang, Ok Hwa; Kim, Se Gun; Shin, Dong Won; Kwon, Dong Yeul.

In: PLoS One, Vol. 9, No. 7, e102697, 21.07.2014.

Research output: Contribution to journalArticle

Choi, Jang Gi ; Mun, Su Hyun ; Chahar, Harendra S. ; Bharaj, Preeti ; Kang, Ok Hwa ; Kim, Se Gun ; Shin, Dong Won ; Kwon, Dong Yeul. / Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. In: PLoS One. 2014 ; Vol. 9, No. 7.
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