Methylamine metabolism to formaldehyde by vascular semicarbazide-sensitive amine oxidase

Paul J. Boor, Margaret B. Trent, Geoffrey A. Lyles, Ming Tao, Ghulam Ansari

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The capacity of the vascular enzyme, semicarbazide-sensitive amine oxidase (SSAO), to metabolize methylamine to the potentially toxic product, formaldehyde, was tested using rat aortic homogenates and purified porcine aortic SSAO. Formaldehyde production in incubations of enzyme source with methylamine (1 mM) was detected by high performance liquid chromatography and product was confirmed by desorption chemical ionization mass spectrometry (DCI-MS). Inhibitor studies using the specific SSAO inhibitor semicarbazide and the monoamine oxidase inhibitor pargyline indicate that SSAO is responsible for metabolism of methylamine to formaldehyde. These results suggest the possibility that elevated methylamine found in several pathologic states (such as uremia and diabetes mellitus), or generated from exogenous sources, could result in overproduction of formaldehyde in tissues with high SSAO activity, especially blood vessels.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalToxicology
Volume73
Issue number3
DOIs
StatePublished - 1992

Fingerprint

Amine Oxidase (Copper-Containing)
Metabolism
Formaldehyde
Blood Vessels
Pargyline
Monoamine Oxidase Inhibitors
Uremia
Poisons
Blood vessels
High performance liquid chromatography
Enzymes
Medical problems
Ionization
Mass spectrometry
Rats
Desorption
Mass Spectrometry
Diabetes Mellitus
Swine
High Pressure Liquid Chromatography

Keywords

  • Aorta
  • Formaldehyde
  • Methylamine
  • Pargyline
  • Semicarbazide
  • Semicarbazide-sensitive amine oxidase

ASJC Scopus subject areas

  • Toxicology

Cite this

Methylamine metabolism to formaldehyde by vascular semicarbazide-sensitive amine oxidase. / Boor, Paul J.; Trent, Margaret B.; Lyles, Geoffrey A.; Tao, Ming; Ansari, Ghulam.

In: Toxicology, Vol. 73, No. 3, 1992, p. 251-258.

Research output: Contribution to journalArticle

Boor, Paul J. ; Trent, Margaret B. ; Lyles, Geoffrey A. ; Tao, Ming ; Ansari, Ghulam. / Methylamine metabolism to formaldehyde by vascular semicarbazide-sensitive amine oxidase. In: Toxicology. 1992 ; Vol. 73, No. 3. pp. 251-258.
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