Mice depleted of αβ but not γδ T cells are resistant to mortality caused by cecal ligation and puncture

Victor T. Enoh, Scott H. Lin, Cheng Y. Lin, Tracy Toliver-Kinsky, Erle D. Murphey, Tushar K. Varma, Edward R. Sherwood

Research output: Contribution to journalArticle

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Abstract

The present study was undertaken to determine whether the mice depleted of αβ or γδ T cells show resistance to acute polymicrobial sepsis caused by cecal ligation and puncture (CLP). T-cell receptor β knockout (βTCRKO) and T-cell receptor δ knockout (δTCRKO) mice were used. An additional group of mice was treated with an antibody against the αβ T-cell receptor to induce αβ T-cell depletion; a subset of αβ T cell-deficient mice was also treated with anti-asialoGM1 to deplete natural killer (NK) cells. The mice underwent CLP and were monitored for survival, temperature, acid-base balance, bacterial counts, and cytokine production. The βTCRKO mice and the wild-type mice treated with anti-β T-cell receptor (anti-TCRβ) antibody showed improved survival after CLP compared with wild-type mice. The treatment of αβ T cell-deficient mice with anti-asialoGM1further improved survival after CLP, especially when the mice were treated with imipenem. The improved survival observed in αβ T cell-deficient mice was associated with less hypothermia, improved acid-base balance, and decreased production of the proinflammatory cytokines interleukin (IL) 6 and macrophage inflammatory protein (MIP) 2. Compared with wild-type controls, the overall survival was not improved in δTCRKO mice. The concentrations of IL-6 and MIP-2 in plasma and cytokine mRNA expression in tissues were not significantly different between wild-type and δTCRKO mice. These studies indicate that mice depleted of αβ but not of γδ T cells are resistant to mortality in an acutely lethal model of CLP. The depletion of NK cells caused further survival benefit in αβ T cell-deficient mice. These findings suggest that αβ T and NK cells mediate or facilitate CLP-induced inflammatory injury.

Original languageEnglish (US)
Pages (from-to)507-519
Number of pages13
JournalShock
Volume27
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Punctures
Ligation
T-Lymphocytes
T-Cell Antigen Receptor
Mortality
Knockout Mice
Chemokine CXCL2
Acid-Base Equilibrium
Cytokines
Natural Killer Cells
Interleukin-6
Natural Killer T-Cells
Antibodies
Bacterial Load
Imipenem
T-Lymphocyte Subsets
Hypothermia
Sepsis
Messenger RNA
Temperature

Keywords

  • Bacteremia
  • Inflammation
  • Lymphocytes
  • Peritonitis
  • Sepsis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Mice depleted of αβ but not γδ T cells are resistant to mortality caused by cecal ligation and puncture. / Enoh, Victor T.; Lin, Scott H.; Lin, Cheng Y.; Toliver-Kinsky, Tracy; Murphey, Erle D.; Varma, Tushar K.; Sherwood, Edward R.

In: Shock, Vol. 27, No. 5, 05.2007, p. 507-519.

Research output: Contribution to journalArticle

Enoh, Victor T. ; Lin, Scott H. ; Lin, Cheng Y. ; Toliver-Kinsky, Tracy ; Murphey, Erle D. ; Varma, Tushar K. ; Sherwood, Edward R. / Mice depleted of αβ but not γδ T cells are resistant to mortality caused by cecal ligation and puncture. In: Shock. 2007 ; Vol. 27, No. 5. pp. 507-519.
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