Micellar electrokinetic capillary chromatographic separation of steroids in urine by trioctylphosphine oxide and cationic surfactant

Mohammad Amin Abubaker, J. R. Petersen, M. G. Bissell

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Separation of the six structurally similar and hydrophobic neutral steroids, testosterone, dimethyltestosterone, testosterone propionate, cortisone, hydrocortisone and 17-deoxycorticosterone, was achieved by hydrophobic micellar elektrokinetic chromatography. A triphasic separation involving micellar dodecyltrimethylammonium bromide (DTAB), a dynamic bilayer formed due to electrostatic interaction between the silica surface and DTAB, and aqueous phase is proposed to account for the observed separation of the steroids. The running buffer consisted of 0.05 M DTAB and 0.0052 M trioctylphosphine oxide in 0.01 M of phosphate buffer pH 7.4. A detection limit of 500 ng/ml was achieved for each steroid and the application of the method to urine samples is described.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume674
Issue number1
DOIs
StatePublished - Dec 1 1995

ASJC Scopus subject areas

  • Chemistry(all)

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