Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides

Per E. Andren, Mark Emmett, Richard M. Caprioli

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H] +3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.

Original languageEnglish (US)
Pages (from-to)867-869
Number of pages3
JournalJournal of the American Society for Mass Spectrometry
Volume5
Issue number9
DOIs
StatePublished - Sep 1994
Externally publishedYes

Fingerprint

Neurotensin
Neuropeptides
Ions
Salt removal
Electrospray ionization
Methionine Enkephalin
Peptides
Peptide Fragments
Ion sources
Needles
Flow rate
Injections

ASJC Scopus subject areas

  • Spectroscopy
  • Structural Biology

Cite this

Micro-Electrospray : Zeptomole/attomole per microliter sensitivity for peptides. / Andren, Per E.; Emmett, Mark; Caprioli, Richard M.

In: Journal of the American Society for Mass Spectrometry, Vol. 5, No. 9, 09.1994, p. 867-869.

Research output: Contribution to journalArticle

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