Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides

Per E. Andren, Mark R. Emmett, Richard M. Caprioli

Research output: Contribution to journalArticlepeer-review

128 Scopus citations


The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.

Original languageEnglish (US)
Pages (from-to)867-869
Number of pages3
JournalJournal of the American Society for Mass Spectrometry
Issue number9
StatePublished - Sep 1994
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy


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