Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots: Understanding the bacterial toxicity of semiconductor nanoparticles

Juan P. Monrás, Bernardo Collao, Roberto C. Molina-Quiroz, Gonzalo A. Pradenas, Luis A. Saona, Vicente Durán-Toro, Nicolás Órdenes-Aenishanslins, Felipe A. Venegas, David E. Loyola, Denisse Bravo, Paulina F. Calderón, Iván L. Calderón, Claudio C. Vásquez, Thomas G. Chasteen, Desiré A. Lopez, José M. Pérez-Donoso

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Most semiconductor nanoparticles used in biomedical applications are made of heavy metals and involve synthetic methods that require organic solvents and high temperatures. This issue makes the development of water-soluble nanoparticles with lower toxicity a major topic of interest. In a previous work our group described a biomimetic method for the aqueous synthesis of CdTe-GSH Quantum Dots (QDs) using biomolecules present in cells as reducing and stabilizing agents. This protocol produces nanoparticles with good fluorescent properties and less toxicity than those synthesized by regular chemical methods. Nevertheless, biomimetic CdTe-GSH nanoparticles still display some toxicity, so it is important to know in detail the effects of these semiconductor nanoparticles on cells, their levels of toxicity and the strategies that cells develop to overcome it. Results: In this work, the response of E. coli exposed to different sized-CdTe-GSH QDs synthesized by a biomimetic protocol was evaluated through transcriptomic, biochemical, microbiological and genetic approaches. It was determined that: i) red QDs (5 nm) display higher toxicity than green (3 nm), ii) QDs mainly induce expression of genes involved with Cd+2 stress (zntA and znuA) and tellurium does not contribute significantly to QDs-mediated toxicity since cells incorporate low levels of Te, iii) red QDs also induce genes related to oxidative stress response and membrane proteins, iv) Cd2+ release is higher in red QDs, and v) QDs render the cells more sensitive to polymyxin B. Conclusion: Based on the results obtained in this work, a general model of CdTe-GSH QDs toxicity in E. coli is proposed. Results indicate that bacterial toxicity of QDs is mainly associated with cadmium release, oxidative stress and loss of membrane integrity. The higher toxicity of red QDs is most probably due to higher cadmium content and release from the nanoparticle as compared to green QDs. Moreover, QDs-treated cells become more sensitive to polymyxin B making these biomimetic QDs candidates for adjuvant therapies against bacterial infections.

Original languageEnglish (US)
Article number1099
JournalBMC Genomics
Volume15
Issue number1
DOIs
StatePublished - Dec 12 2014
Externally publishedYes

Fingerprint

Quantum Dots
Microarray Analysis
Escherichia coli
Biomimetics
Nanoparticles
Polymyxin B
Cadmium
Oxidative Stress
Tellurium
Excipients
Reducing Agents
Heat-Shock Proteins
Heavy Metals

Keywords

  • Cadmium
  • Nanoparticles
  • Oxidative stress
  • Toxicity mechanism
  • Transcriptomic response

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Monrás, J. P., Collao, B., Molina-Quiroz, R. C., Pradenas, G. A., Saona, L. A., Durán-Toro, V., ... Pérez-Donoso, J. M. (2014). Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots: Understanding the bacterial toxicity of semiconductor nanoparticles. BMC Genomics, 15(1), [1099]. https://doi.org/10.1186/1471-2164-15-1099

Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots : Understanding the bacterial toxicity of semiconductor nanoparticles. / Monrás, Juan P.; Collao, Bernardo; Molina-Quiroz, Roberto C.; Pradenas, Gonzalo A.; Saona, Luis A.; Durán-Toro, Vicente; Órdenes-Aenishanslins, Nicolás; Venegas, Felipe A.; Loyola, David E.; Bravo, Denisse; Calderón, Paulina F.; Calderón, Iván L.; Vásquez, Claudio C.; Chasteen, Thomas G.; Lopez, Desiré A.; Pérez-Donoso, José M.

In: BMC Genomics, Vol. 15, No. 1, 1099, 12.12.2014.

Research output: Contribution to journalArticle

Monrás, JP, Collao, B, Molina-Quiroz, RC, Pradenas, GA, Saona, LA, Durán-Toro, V, Órdenes-Aenishanslins, N, Venegas, FA, Loyola, DE, Bravo, D, Calderón, PF, Calderón, IL, Vásquez, CC, Chasteen, TG, Lopez, DA & Pérez-Donoso, JM 2014, 'Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots: Understanding the bacterial toxicity of semiconductor nanoparticles', BMC Genomics, vol. 15, no. 1, 1099. https://doi.org/10.1186/1471-2164-15-1099
Monrás, Juan P. ; Collao, Bernardo ; Molina-Quiroz, Roberto C. ; Pradenas, Gonzalo A. ; Saona, Luis A. ; Durán-Toro, Vicente ; Órdenes-Aenishanslins, Nicolás ; Venegas, Felipe A. ; Loyola, David E. ; Bravo, Denisse ; Calderón, Paulina F. ; Calderón, Iván L. ; Vásquez, Claudio C. ; Chasteen, Thomas G. ; Lopez, Desiré A. ; Pérez-Donoso, José M. / Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots : Understanding the bacterial toxicity of semiconductor nanoparticles. In: BMC Genomics. 2014 ; Vol. 15, No. 1.
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AU - Pradenas, Gonzalo A.

AU - Saona, Luis A.

AU - Durán-Toro, Vicente

AU - Órdenes-Aenishanslins, Nicolás

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AU - Bravo, Denisse

AU - Calderón, Paulina F.

AU - Calderón, Iván L.

AU - Vásquez, Claudio C.

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AU - Lopez, Desiré A.

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