Microbial growth patterns in intravenous fat emulsions

D. Keammerer, C. G. Mayhall, G. O. Hall, L. J. Pesko, R. B. Thomas

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Microbial growth patterns were studied in intravenous fat emulsions under conditions that simulated touch contamination before or during administration. Commercially available emulsions of 10% and 20% soybean oil and 10% safflower oil in 500-ml bottles were inoculated with two concentrations of each of four organisms; a coagulase-negative Staphylococcus isolated from a venipuncture site, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. The bottles were kept at room temperature, and samples were taken by direct puncture of the i.v. port at 0, 3, 6, 12, 24, 48, and 72 hours, diluted, and plated. Emulsions were visually inspected daily. Growth of the coagulase-negative Staphylococcus was minimal for 48 hours. E. coli showed substantial growth within 12 hours in all three emulsions. Growth patterns for Ps. aeruginosa were similar in all emulsions, and growth approximated that of E. coli within 48 hours. The growth rate of C. albicans was intermediate between that of Staphylococcus and those of E. coli and Ps. aeruginosa. Growth of C. albicans was greater in 10% safflower oil emulsion than in the other emulsions. No physical changes were observed. The coagulase-negative Staphylococcus showed less growth than C. albicans, Ps. aeruginosa, and E. coli. Substantial growth within 12 hours was seen only with E. coli. C. albicans exhibited preferential growth in 10% safflower oil emulsion.

Original languageEnglish (US)
Pages (from-to)1650-1653
Number of pages4
JournalAmerican Journal of Hospital Pharmacy
Volume40
Issue number10
StatePublished - 1983
Externally publishedYes

Fingerprint

Intravenous Fat Emulsions
Emulsions
Growth
Candida albicans
Safflower Oil
Escherichia coli
Staphylococcus
Pseudomonas aeruginosa
Coagulase
Soybean Oil
Phlebotomy
Touch
Punctures

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Leadership and Management

Cite this

Keammerer, D., Mayhall, C. G., Hall, G. O., Pesko, L. J., & Thomas, R. B. (1983). Microbial growth patterns in intravenous fat emulsions. American Journal of Hospital Pharmacy, 40(10), 1650-1653.

Microbial growth patterns in intravenous fat emulsions. / Keammerer, D.; Mayhall, C. G.; Hall, G. O.; Pesko, L. J.; Thomas, R. B.

In: American Journal of Hospital Pharmacy, Vol. 40, No. 10, 1983, p. 1650-1653.

Research output: Contribution to journalArticle

Keammerer, D, Mayhall, CG, Hall, GO, Pesko, LJ & Thomas, RB 1983, 'Microbial growth patterns in intravenous fat emulsions', American Journal of Hospital Pharmacy, vol. 40, no. 10, pp. 1650-1653.
Keammerer D, Mayhall CG, Hall GO, Pesko LJ, Thomas RB. Microbial growth patterns in intravenous fat emulsions. American Journal of Hospital Pharmacy. 1983;40(10):1650-1653.
Keammerer, D. ; Mayhall, C. G. ; Hall, G. O. ; Pesko, L. J. ; Thomas, R. B. / Microbial growth patterns in intravenous fat emulsions. In: American Journal of Hospital Pharmacy. 1983 ; Vol. 40, No. 10. pp. 1650-1653.
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