Microbial load of umbilical cord blood Ureaplasma species and Mycoplasma hominis in preterm prelabor rupture of membranes

Marian Kacerovsky, Lenka Pliskova, Ramkumar Menon, Radka Kutova, Ivana Musilova, Jan Maly, Ctirad Andrys

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: To evaluate Ureaplasma species and M. hominis DNA in the umbilical cord blood and its correlation with its microbial load in the amniotic fluid, as a measure of microbial burden in fetal inflammatory response and neonatal outcome in pregnancies complicated by preterm prelabor rupture of membranes (pPROM). Study design: A retrospective study of 158 women with singleton pregnancies complicated by pPROM between 240/7 and 366/7 weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis, and umbilical cord blood was obtained by venipuncture from umbilical cords immediately after the delivery of the neonates. The Ureaplasma species and M. hominis DNA was quantitated using absolute quantification techniques. Result: Ureaplasma species and M. hominis DNA was identified in 9% of the umbilical cord blood samples. No correlation between the amniotic fluid and umbilical cord blood microbial load was observed. The presence of Ureaplasma species and M. hominis DNA in the umbilical cord blood had no impact on short-term neonatal morbidity. Conclusions: A high microbial load of genital mycoplasma Ureaplasma species DNA in the umbilical cord in pregnancies complicated by pPROM is not associated with a high fetal inflammatory response and is therefore not associated with serious neonatal morbidity.

Original languageEnglish (US)
Pages (from-to)1627-1632
Number of pages6
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume27
Issue number16
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

Fingerprint

Mycoplasma hominis
Ureaplasma
Fetal Blood
Rupture
Amniotic Fluid
Membranes
DNA
Umbilical Cord
Morbidity
Pregnancy
Phlebotomy
Amniocentesis
Mycoplasma
Pregnancy Outcome
Retrospective Studies
Newborn Infant

Keywords

  • Amniotic fluid
  • Fetal inflammatory response
  • Genital mycoplasmas

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Microbial load of umbilical cord blood Ureaplasma species and Mycoplasma hominis in preterm prelabor rupture of membranes. / Kacerovsky, Marian; Pliskova, Lenka; Menon, Ramkumar; Kutova, Radka; Musilova, Ivana; Maly, Jan; Andrys, Ctirad.

In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 27, No. 16, 01.11.2014, p. 1627-1632.

Research output: Contribution to journalArticle

Kacerovsky, Marian ; Pliskova, Lenka ; Menon, Ramkumar ; Kutova, Radka ; Musilova, Ivana ; Maly, Jan ; Andrys, Ctirad. / Microbial load of umbilical cord blood Ureaplasma species and Mycoplasma hominis in preterm prelabor rupture of membranes. In: Journal of Maternal-Fetal and Neonatal Medicine. 2014 ; Vol. 27, No. 16. pp. 1627-1632.
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