Microbiota dysbiosis and its pathophysiological significance in bowel obstruction

Shrilakshmi Hegde, You Min Lin, George Golovko, Kamil Khanipov, Yingzi Cong, Tor Savidge, Yuriy Fofanov, Xuan-Zheng Shi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bowel obstruction (OB) causes local and systemic dysfunctions. Here we investigated whether obstruction leads to alterations in microbiota community composition and total abundance, and if so whether these changes contribute to dysfunctions in OB. Partial colon obstruction was maintained in rats for 7 days. The mid colon and its intraluminal feces - proximal to the obstruction - were studied. OB did not cause bacterial overgrowth or mucosa inflammation, but induced profound changes in fecal microbiota composition and diversity. At the phylum level, the 16S rRNA sequencing showed a significant decrease in the relative abundance of Firmicutes with corresponding increases in Proteobacteria and Bacteroidetes in OB compared with sham controls. Daily treatment using broad spectrum antibiotics dramatically reduced total bacterial abundance, but increased the relative presence of Proteobacteria. Antibiotics eliminated viable bacteria in the spleen and liver, but not in the mesentery lymph node in OB. Although antibiotic treatment decreased muscle contractility in sham rats, it had little effect on OB-associated suppression of muscle contractility or inflammatory changes in the muscle layer. In conclusion, obstruction leads to marked dysbiosis in the colon. Antibiotic eradication of microbiota had limited effects on obstruction-associated changes in inflammation, motility, or bacterial translocation.

Original languageEnglish (US)
Article number13044
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Dysbiosis
Microbiota
Anti-Bacterial Agents
Proteobacteria
Colon
Muscles
Bacteroidetes
Inflammation
Bacterial Translocation
Mesentery
Feces
Mucous Membrane
Spleen
Lymph Nodes
Bacteria
Liver

ASJC Scopus subject areas

  • General

Cite this

Microbiota dysbiosis and its pathophysiological significance in bowel obstruction. / Hegde, Shrilakshmi; Lin, You Min; Golovko, George; Khanipov, Kamil; Cong, Yingzi; Savidge, Tor; Fofanov, Yuriy; Shi, Xuan-Zheng.

In: Scientific Reports, Vol. 8, No. 1, 13044, 01.12.2018.

Research output: Contribution to journalArticle

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