Microbiota metabolite short-chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43

W. Wu, M. Sun, F. Chen, A. T. Cao, H. Liu, Y. Zhao, Xiangsheng Huang, Y. Xiao, Suxia Yao, Q. Zhao, Z. Liu, Yingzi Cong

Research output: Contribution to journalArticle

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Abstract

Intestinal IgA, which is regulated by gut microbiota, has a crucial role in maintenance of intestinal homeostasis and in protecting the intestines from inflammation. However, the means by which microbiota promotes intestinal IgA responses remain unclear. Emerging evidence suggests that the host can sense gut bacterial metabolites in addition to pathogen-associated molecular patterns and that recognition of these small molecules influences host immune response in the intestines and beyond. We reported here that microbiota metabolite short-chain fatty acid acetate promoted intestinal IgA responses, which was mediated by "metabolite-sensing" GPR43. GPR43 -/- mice demonstrated lower levels of intestinal IgA and IgA + gut bacteria compared with those in wild type (WT) mice. Feeding WT but not GPR43 -/- mice acetate but not butyrate promoted intestinal IgA response independent of T cells. Acetate promoted B-cell IgA class switching and IgA production in vitro in the presence of WT but not GPR43 -/- dendritic cells (DCs). Mechanistically, acetate-induced DC expression of Aldh1a2, which converts Vitamin A into its metabolite retinoic acid (RA). Moreover, blockade of RA signaling inhibited the acetate induction of B-cell IgA production. Our studies thus identified a new pathway by which microbiota promotes intestinal IgA response through its metabolites.

Original languageEnglish (US)
Pages (from-to)946-956
Number of pages11
JournalMucosal Immunology
Volume10
Issue number4
DOIs
StatePublished - Jul 1 2017

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Volatile Fatty Acids
Microbiota
Immunoglobulin A
Acetates
Tretinoin
Dendritic Cells
Intestines
B-Lymphocytes
Immunoglobulin Class Switching
Butyrates
Vitamin A
Homeostasis
Maintenance
Inflammation
Bacteria
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Microbiota metabolite short-chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43. / Wu, W.; Sun, M.; Chen, F.; Cao, A. T.; Liu, H.; Zhao, Y.; Huang, Xiangsheng; Xiao, Y.; Yao, Suxia; Zhao, Q.; Liu, Z.; Cong, Yingzi.

In: Mucosal Immunology, Vol. 10, No. 4, 01.07.2017, p. 946-956.

Research output: Contribution to journalArticle

Wu, W. ; Sun, M. ; Chen, F. ; Cao, A. T. ; Liu, H. ; Zhao, Y. ; Huang, Xiangsheng ; Xiao, Y. ; Yao, Suxia ; Zhao, Q. ; Liu, Z. ; Cong, Yingzi. / Microbiota metabolite short-chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43. In: Mucosal Immunology. 2017 ; Vol. 10, No. 4. pp. 946-956.
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